pU-VEGF-siRNA对人骨髓间质干细胞的影响  

Effect of pU-VEGF-siRNA on human bone mesenchymal stem cells

作　　者：杨宏辉[1] 高传玉[1] 陈岩[1] 朱中玉[1] 李牧蔚[1] 王现青[1] 牛振民[1] 刘洪智[1] 张静[1] 

机构地区：[1]河南省人民医院心内科,河南郑州450003

出　　处：《中国老年学杂志》2012年第19期4199-4201,共3页Chinese Journal of Gerontology

基　　金：河南省科技攻关课题(112102310221);河南省中青年科技创新人才基金;河南省卫生厅5451工程基金资助

摘　　要：目的观察pU-VEGF-siRNA真核表达载体转染骨髓间质干细胞后血管内皮生长因子(VEGF)mRNA表达及VEGF蛋白质的变化。方法脂质体介导pU-VEGF-siRNA质粒转染体外培养的人骨髓间质干细胞,RT-PCR方法检测转染后骨髓间质干细胞VEGF mRNA表达水平,免疫印迹法半定量检测转染后骨髓间质干细胞VEGF蛋白质水平。结果荧光显微镜提示脂质体介导质粒转染人骨髓间质干细胞成功,且效率较高;与对照组相比,VEGF mRNA及蛋白质表达水平在pU-VEGF-siRNA转染组显著降低,空载体转染组无明显变化。结论 pU-VEGF-siRNA质粒转染骨髓间质干细胞,可降低骨髓间质干细胞VEGF mRNA表达水平,下调其VEGF蛋白质水平。Objective To investigate the expressions of vascular endothelial growth factor （VEGF） mRNA and protein in bone mes- enchymal stem cells （BMSCs） transfected with pU-VEGF-siRNA. Methods pU-VEGF-siRNA was transfected into BMSCs by liposome. Expression of VEGF mRNA in BMSCs was detected by RT-PCR. Expression of VEGF protein in BMSCs was detected by Western blot. Re- suits pU-VEGF-siRNA was transfected into BMSCs by liposome successfully and the efficiency was high. Compared with control group, ex- pressions of VEGF mRNA and protein were lower in transfected group and had no change in empty vector transfected group. Conclusions Transfecting pU-VEGF-siRNA into BMSCs could decrease the expressions of VEGF mRNA and protein.

关 键 词：血管内皮生长因子 SIRNA 骨髓间质干细胞 转染 

分 类 号：R34[医药卫生—基础医学]