低温预处理与光照条件对蓖麻花药愈伤组织诱导的影响  

Effects of Cold Pretreatment and Light Conditions on Castor Anther Callus Induction

作　　者：邵志敏[1,2] 陈永胜[1,2] 黄凤兰[1,2] 李国瑞 栾世慧[1] 彭木[1] 王文跃[1] 陈丽丽[1] 王丹[3] 

机构地区：[1]内蒙古民族大学,内蒙古通辽028000 [2]内蒙古高校蓖麻产业工程技术中心,内蒙古通辽028000 [3]内蒙古通辽市农业科学研究院,内蒙古通辽028000

出　　处：《内蒙古民族大学学报（自然科学版）》2012年第2期189-193,共5页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：国家自然科学基金项目(31060194);教育部新世纪人才支持计划资助项目(NCET-0870)

摘　　要：为进一步优化蓖麻花药愈伤诱导培养条件,本试验以通蓖5号、通蓖9号和哲蓖4号三个不同品种的蓖麻花药为试验材料,研究了低温预处理与光照条件对蓖麻花药愈伤组织诱导的影响.结果表明:三个品种的蓖麻花药经4℃低温预处理5d均可显著提高愈伤组织诱导率,其中以哲蓖4号为最高,愈伤诱导率达到22.55%.黑暗处理较光照处理更有利于蓖麻花药愈伤组织的诱导,其中以暗处理15d诱导效果最佳.The castor anthers of Tongbi No.5, Tongbi No.9 and zhebi No.4 three different varieties were cultured under low-temperature pretreatment and light conditions to study the effects on the castor anther callus induction for further optimize culture conditions of castor anther callus induction. The results show that： three varieties of castor anther all can significantly improve the callus induction rate after four^C low temperature pretreatment 5 days, in which zhebi No.4 had the highest callus induction rate, which reached 22.55%. Compared with light conditions, dark treatment were suitable for anther callus induction, in which dark treatment for 15 days had the best induction results.

关 键 词：蓖麻 花药培养 低温预处理 光照条件 愈伤组织 褐化 

分 类 号：S565.6[农业科学—作物学]