终末期肾病自然杀伤细胞的活性及其对白细胞介素2的反应  

NK cell function in end stage renal disease patients: a flow cytometry assay and its response to IL-2 activity

作　　者：程庆(王乐)[1] 陈香美[1] 刘成贵[2] 李岩[2] 

机构地区：[1]中国人民解放军军医进修学院肾内科 [2]中国人民解放军军医进修学院肿瘤单克隆研究室

出　　处：《军医进修学院学报》1990年第2期141-143,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：应用流式细胞仪检测了13例终末期肾病(ESRD)患者的外周血自然杀伤细胞(NK细胞)活性及其对白细胞介素2(IL-2)调节的反应,发现ESRD患者的NK细胞活性明显降低,IL-2对患者的NK细胞调节作用强弱不一,荧光标记的单克隆抗体CD_(16)与NK细胞活性相关显著。To investigate the Natural Killer (NK) cell activity of peripheral blood in the end-stage renal disease renal disease (ESRD) patients, a flow cytometry assay based on the cytotoxic action on target cells was performed for specimen obtaind from 13 cases on chronic hemodialysis. The result showed a marked decrement of NK cell activity in these patients as compared with the control (12.36 ± 5.09 vs 17.84 ± 3.71 P<0.001). The response of NK cell in this group of patients to IL-2 stimulation was also measured. Obviously, in normal control there was an increase of cytotoxic action of these cells after the addition of IL-2 into the culture media. Yet it was not present in the ESRD. It indicated that both the activity of NK cell and their response to IL-2 were attenuated markedly in ESRD, which is in accordance with the high susceptibility to malignancy and infection of these patients. Recently, NK cell can also be recognized by its surface antigen CD16(Leull). Using FITC Labelled anti CD16 McAb and flow cytometry technique, we enumerated the number of NK cell in the specimen from this group of patients. The results were comparable to that assessed by the NK cell activity as outlined in this paper.

关 键 词：有丝分裂因子 淋巴细胞 自然杀伤细胞 肾疾病 

分 类 号：R692.03[医药卫生—泌尿科学]