丙氨酰-谷氨酰胺投喂方式对建鲤生长、抗氧化及免疫力的影响  

EFFECT OF ALA-GLN FEEDING ON GROWTH, ANTIOXIDANT LEVEL,AND IMMUNITY OF CYPRINUS CARPIO VAR. JIAN

作　　者：王桂芹[1] 韩宇田[1] 芦洪梅 牛小天[1] 李子平[1] 何义进[3] 赵朝阳[3] 秦贵信[1] 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]长春通威饲料有限公司,长春130456 [3]中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,无锡214081

出　　处：《水生生物学报》2012年第5期837-842,共6页Acta Hydrobiologica Sinica

基　　金：中国博士后基金资助(200763016);农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金(BZ2009-01)资助

摘　　要：以初始质量为(33.52±0.17)g建鲤鱼种为研究对象,在室内单循环养殖系统中进行8周(w)生长试验,分别配制添加0.0%(对照饲料)和0.5%(试验饲料)丙氨酰-谷氨酰胺(Ala-Gln)的等氮(35 g/kg粗蛋白)、等能(17kJ/g能量)饲料,采用5种不同的Ala-Gln投喂方式[连续8w投喂对照饲料(I,对照组);试验饲料2w交替投喂(II);前4w投喂试验饲料、后4w投喂对照饲料(III);前4w投喂对照饲料、后4w投喂试验饲料(IV);8w连续投喂试验饲料(V)],探讨Ala-Gln投喂方式对建鲤生长、抗氧化及免疫力的影响。结果表明:Ala-Gln连续投喂和不连续投喂的生长都显著高于对照组(P<0.05)。2w交替投喂的生长率显著高于4w交替和连续8w投喂试验饲料组(P<0.05),前4w投喂试验饲料且后4w投喂对照饲料的生长率要高于8w连续投喂试验饲料组(P<0.05)。后4w投喂试验饲料组和连续8w投喂试验饲料组的血清SOD显著高于对照组(P<0.05);连续8w投喂试验饲料组显著高于2w交替投喂试验饲料组和前4w投喂试验饲料组的血清SOD(P<0.05)。Ala-Gln各种投喂方式组的肝胰脏谷胱甘肽过氧化物酶(GSH-Px)都显著高于对照组(P<0.05)。后4w投喂试验饲料组和连续8w投喂试验饲料组的血清GSH-Px显著高于对照组(P<0.05)。在Ala-Gln四种投喂方式组中,除2w交替投喂组外,其他三种投喂方式组的头肾LZM都显著高于对照组(P<0.05)。后4w投喂试验饲料组和8w连续投喂试验饲料组的脾脏LZM都显著高于对照组(P<0.05)。对照组头肾NO要显著高于各投喂方式组(P<0.05)。在试验条件下,饲料中添加Ala-Gln可提高建鲤的生长、抗氧化和免疫力。不同投喂方式间亦有显著差异,从生长、抗氧化和免疫的角度,结合经济性和适用性等进行考虑,建议采用2w间隔投喂的方式。This study was carried out using healthy Cyprinus with the initial body weight or （33.52±0.17）g m a single recirculating system for 8 weeks. Diets with equal levels of protein （35 g/kg） and energy （17 kJ/g） were administered to the Cyprinus at concentrations of 0.0 % （control diet） and 0.5% （trial diet） Ala-Gln of dietary dry matter in five different feeding modes： continuous 8 weeks of control diet （group I or control）, trial diet at an interval of 2 weeks （group II）, trial diet at 4 weeks and then control diet at 4 weeks （group III）, the contrary to group III （group IV） and continuous 8-week trial diet （group V）. The trail was conducted to investigate the effects of different feeding mode of Ala-Gln on growth, immunity and antioxidant in Cyprinus. Our results indicated that growth rates under all different feeding modes of Ala-Gln were significantly higher than that of the control group （P〈0.05）. Growth rate of II was higher than that of III, IV, V groups （P〈0.05）, and growth rate of III was higher than that of V group （P〈0.05）. Serum SOD of the 1V and V groups were significantly higher than the control group （P〈0.05）, and serum SOD of the V group was significantly higher than those of II and III groups （P〈0.05）. GSH-Px in hepatopancreas of different feeding modes were signifi- cantly higher than that of the control group （P〈0.05）, Serum GSH-Px of IV and V groups were higher than that of the control group （P〈0.05）. LZM in the head kidneys of the Ala-Gln groups were higher than the control group, only group II showed no significant difference （P〉0.05）, LZM in the spleen of the groups IV and V were significantly higher than that of the control group （P〈0.05）. In the current experiment, we concluded that adding Ala-Gln could improve the growth, immunity and antioxidant levels of Cyprinus. There were significant differences of different feeding modes. Given economical efficiency and practicability, feeding mode with 2-

关 键 词：丙氨酰-谷氨酰胺 建鲤 生长 抗氧化 免疫力 

分 类 号：S965.116[农业科学—水产养殖]