细胞色素c在TiO_2纳米粒子表面的增强拉曼光谱研究  

Enhanced Raman scatter study of cytochrome c absorbed on TiO_2 nanoparticles

作　　者：李志士[1] 赵冰[1] 

机构地区：[1]吉林大学超分子结构与材料国家重点实验室,长春130012

出　　处：《中国科技论文》2012年第9期674-678,共5页China Sciencepaper

基　　金：国家自然科学基金资助项目(20473029;20573041;20873050;20921003;20973074);教育部引智计划资助项目(B06009);高等学校博士学科点专项科研基金资助项目(20110061110017)

摘　　要：TiO2纳米粒子具有良好的光学活性、催化活性及生物相容性。在TiO2纳米粒子表面可观测到Cyt c分子增强的拉曼信号。同Cyt c本体的拉曼光谱对比得知,吸附在TiO2表面的Cyt c分子的光谱有所不同,某些峰发生位移,某些峰的相对强度发生变化。同时,调节Cytc的浓度变化范围从10-4mol/L至5×10-7mol/L,发现了Cyt c分子增强拉曼光谱的浓度依赖性。吸附在TiO2纳米粒子表面的Cytc检测限可以达到5×10-6mol/L。In this work,enhanced Raman spectra of cytochrome c（Cyt c） absorbed on TiO2nanoparticles have been observed.Raman spectra are quite different on Cyt c bulk according to the interaction between Cyt c and TiO2nanoparticles.The positions of some peaks shift and the relative intensities of some peaks change as well.For the quantitative analysis,the concentration of Cyt c is adjusted from 10-4mol/L to 5×10-7mol/L.Aconcentration-dependent relationship with intensity is found.Meanwhile,the detection limit of Cyt c can achieve as low as 5×10-6mol/L.It is possible to carry out semi-quantitative analysis of the concentration of Cytc.

关 键 词：表面增强拉曼光谱 TIO2 细胞色素C 半导体纳米粒子 

分 类 号：TB383.1[一般工业技术—材料科学与工程]