Study on In vitro Regeneration System of Melon  

作　　者：Yan ZHAO Qingbo LIU Chunmei REN Shanshan MA Hongmei HUANG 

机构地区：[1]College of Bioscience and Biotechnology,Hunan Agricultural University

出　　处：《Agricultural Biotechnology》2012年第1期23-26,共4页农业生物技术（英文版）

基　　金：Supported by the Foundation of Hunan Provincial Department of Science and Technology(2010FJ3103)

摘　　要：[ Objective] This study aimed to optimize the in vitro regeneration system of melon. [ Method] Melon variety Nanxiang91023 was selected as experi- mental materials, with the cotyledons and hypocotyls as explants, different types and concentrations of growth regulators were supplemented at different stages of tis- sue culture, to explore the simple and effective medium formula for regeneration of melon. [ Result] MS ＋ 1.5 mg/L of 6-BA ＋0.2 mg/L of LAA was the optimal medium for induction and proliferation of callus; MS ＋ 1.0 mg/L of 6-BA ＋ 0. 2 mg/L of 2, 4-D was the optimal medium for differentiation of adventitious buds; MS ＋ 1.0 mg/L of ZT ＋ 0.2 mg/L of LAA was the optimal medium for rooting of seedlings. On the basis of above conditions, melon seedlings had high roofing rate and strong roots. [ Conclusion] This study provided a guarantee for the further genetic transformation of improved melon varieties.[ Objective] This study aimed to optimize the in vitro regeneration system of melon. [ Method] Melon variety Nanxiang91023 was selected as experi- mental materials, with the cotyledons and hypocotyls as explants, different types and concentrations of growth regulators were supplemented at different stages of tis- sue culture, to explore the simple and effective medium formula for regeneration of melon. [ Result] MS ＋ 1.5 mg/L of 6-BA ＋0.2 mg/L of LAA was the optimal medium for induction and proliferation of callus; MS ＋ 1.0 mg/L of 6-BA ＋ 0. 2 mg/L of 2, 4-D was the optimal medium for differentiation of adventitious buds; MS ＋ 1.0 mg/L of ZT ＋ 0.2 mg/L of LAA was the optimal medium for rooting of seedlings. On the basis of above conditions, melon seedlings had high roofing rate and strong roots. [ Conclusion] This study provided a guarantee for the further genetic transformation of improved melon varieties.

关 键 词：MELON Regeneration system HYPOCOTYLS COTYLEDONS 

分 类 号：S652[农业科学—果树学]