A Widely Applicable Method for Plant Genomic DNA Extraction  

作　　者：Bin LI Lixia YU Junjun XI Yaping ZHANG Zerui GAO Bo YAN 

机构地区：[1]Southwest Forestry University [2]Yunnan Academy of Tobacco Science

出　　处：《Agricultural Biotechnology》2012年第1期29-30,共2页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31060042);Applied Basic Research Project of Yunnan Province(2009ZC079M)

摘　　要：[Objective] This study aimed to explore a method for plant genomic DNA extraction, to provide guidance for rapid extraction of genomic DNA from plant tissues. [ Method] Based on the published genomic DNA extraction methods, operation steps, reagent amount and processing time of SDS extraction method were optimized. [Result] A widely applicable method was established initially for genomic DNA extraction from various varieties of plants and various kinds of plant tissues. Quality of the extracted genomic DNA was relatively good, which meets the requirements for further operation. [ Conclusion] This study provided guidance for rapid plant genomic DNA extraction.[Objective] This study aimed to explore a method for plant genomic DNA extraction, to provide guidance for rapid extraction of genomic DNA from plant tissues. [ Method] Based on the published genomic DNA extraction methods, operation steps, reagent amount and processing time of SDS extraction method were optimized. [Result] A widely applicable method was established initially for genomic DNA extraction from various varieties of plants and various kinds of plant tissues. Quality of the extracted genomic DNA was relatively good, which meets the requirements for further operation. [ Conclusion] This study provided guidance for rapid plant genomic DNA extraction.

关 键 词：Plant genome DNA extraetion Optimized SDS extraction method 

分 类 号：Q943[生物学—植物学]