Application of Ramp to Improve the Efficiency of RAPD in Longan  

作　　者：Zhiqiang MEI Chunhong LI Chenggang DUAN Xiaoyan LIU 

机构地区：[1]Research Center for Preclinical Medicine,Luzhou Medical College [2]Research Center for Drug and Functional Food,Luzhou Medical College

出　　处：《Agricultural Biotechnology》2012年第1期34-36,50,共4页农业生物技术（英文版）

摘　　要：[ Objective] This study aimed to explore a modified method for improving the efficiency of RAPD technology. [ Method] Longans collected from Si- chuan, Fujian, Guangxi, Guangdong and Hainan were used as experimental materials. Total genomic DNA was extracted from langan leaves with modified CTAB method. 18 random primers were selected for RAPD amplification with ramp rates of 0.3 and 3.0℃/s, respectively. Effects of different ramp rates on RAPD am- plification were compared and the relationship among longan samples was analyzed. [ Result] The resolution and yield could be improved significantly by declining the ramp rate from 3.0 to 0.3℃/s. Cluster analysis showed that similarity coefficient of the five varieties of longan ranged from 0.69 to 0.76. [ Conclusion] This study had confirmed that reducing ramp rate could significantly increase the resolution and yield of RAPD technology, which had provided a new method to improve the efficiency of RAPD.[ Objective] This study aimed to explore a modified method for improving the efficiency of RAPD technology. [ Method] Longans collected from Si- chuan, Fujian, Guangxi, Guangdong and Hainan were used as experimental materials. Total genomic DNA was extracted from langan leaves with modified CTAB method. 18 random primers were selected for RAPD amplification with ramp rates of 0.3 and 3.0℃/s, respectively. Effects of different ramp rates on RAPD am- plification were compared and the relationship among longan samples was analyzed. [ Result] The resolution and yield could be improved significantly by declining the ramp rate from 3.0 to 0.3℃/s. Cluster analysis showed that similarity coefficient of the five varieties of longan ranged from 0.69 to 0.76. [ Conclusion] This study had confirmed that reducing ramp rate could significantly increase the resolution and yield of RAPD technology, which had provided a new method to improve the efficiency of RAPD.

关 键 词：RAPD RAMP LONGAN EFFICIENCY 

分 类 号：S667.2[农业科学—果树学]