Chromosomal Karyotypes of Medicinal Plant Gynura medica  

作　　者：Sanwei FAN Lihui DONG Qingzhi LING Xiaowei TU Zhi LIN Lili GAO Zhaojun WEI 

机构地区：[1]Zhejiang Pharmaceutical College [2]School of Biotechnology and Food Engineering,Hefei University of Technology

出　　处：《Agricultural Biotechnology》2012年第3期15-17,共3页农业生物技术（英文版）

基　　金：Supported by Chinese Medical Science and Technology Program of Zhejiang Province(2011ZB132);the Science and Technology Innovation Program for Undergraduate Students of Zhejiang Province(ZJRCKJCX201007)

摘　　要：[ Objective] This study aimed at investigating the chromosomal characteristics and karyotypes of Gynura medica. [ Method ] Stem tips of G. medica seedlings were taken every 20 min from 6：30 to 10：30 am, and then were treated and squashed. The slices were observed under a microscope for investigating the chromosomal number and karyotypes of the cells in metaphase in which the chromosomes were well dispersed. [ Result] In the total 102 stem tip cells in meta- phase, 96.08% of cells were with 20 chromosomes, and 3.92% of cells were with 40 chromosomes. Optimal time for sampling was from 7：50 to 9：30 am, when more cells were in metaphase. [Conclusion] C. medica is the diploid plant with 20 chromosomes. Karyotype analysis gets formula 2n = 2x = 20m, and all 10 pairs of chromosomes belong to type 1A. The article reporls the chromosomes characteristics of G. rnedica firstly.[ Objective] This study aimed at investigating the chromosomal characteristics and karyotypes of Gynura medica. [ Method ] Stem tips of G. medica seedlings were taken every 20 min from 6：30 to 10：30 am, and then were treated and squashed. The slices were observed under a microscope for investigating the chromosomal number and karyotypes of the cells in metaphase in which the chromosomes were well dispersed. [ Result] In the total 102 stem tip cells in meta- phase, 96.08% of cells were with 20 chromosomes, and 3.92% of cells were with 40 chromosomes. Optimal time for sampling was from 7：50 to 9：30 am, when more cells were in metaphase. [Conclusion] C. medica is the diploid plant with 20 chromosomes. Karyotype analysis gets formula 2n = 2x = 20m, and all 10 pairs of chromosomes belong to type 1A. The article reporls the chromosomes characteristics of G. rnedica firstly.

关 键 词：Gynura reedica CHROMOSOME KARYOTYPE 

分 类 号：S567.239[农业科学—中草药栽培]