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作 者:Guanglin PENG Xiuchen XIN Yuanyuan CHE Zhifen ZHANG Shuyi SHI
机构地区:[1]Yantai Institute,China Agricultural University
出 处:《Agricultural Biotechnology》2012年第3期20-22,共3页农业生物技术(英文版)
基 金:Supported by China Agricultural University (Yantai) Project(yt2007.14)
摘 要:[ Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to sdect callus induction, subculture and rooting media for rapid propagation of H. hybrida.[ Result ] MS + 2.0 - 3.0 mg/L 6-BA + 0.2 - 0.3 mg/L NAA, MS + 1.0 - 2.0 mg/L 6-BA + 0.1 - 0.2 mg/L NAA, MS and 1/2MS + 0.2 mg/L NAA were the appropriate me- dium for callus induction, subculture and rooting, respectively. [ Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technieal reference for industrialized production of H. hybrida.[ Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to sdect callus induction, subculture and rooting media for rapid propagation of H. hybrida.[ Result ] MS + 2.0 - 3.0 mg/L 6-BA + 0.2 - 0.3 mg/L NAA, MS + 1.0 - 2.0 mg/L 6-BA + 0.1 - 0.2 mg/L NAA, MS and 1/2MS + 0.2 mg/L NAA were the appropriate me- dium for callus induction, subculture and rooting, respectively. [ Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technieal reference for industrialized production of H. hybrida.
关 键 词:Hemerocallis hybrida PEDICEL in vitro culture Rapid propagation
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