针刺眼针穴区与非穴区对D-IBS模型大鼠结肠组织VIP及AQP8表达的影响  

Effects of acupoint area and non-acupoint area of eye-acupuncture on expressions of VIP and AQP 8 in colonic tissues in rats with D-IBS

作　　者：刘慧慧[1] 刘旭东[1] 王艳杰[1] 关洪全[1] 柴纪严[1] 赵金茹[1] 王德山[1] 

机构地区：[1]辽宁中医药大学基础医学院.沈阳110847

出　　处：《中国针灸》2012年第10期919-924,共6页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划(973计划)资助项目:2007CB 512702

摘　　要：目的:探讨眼针穴区的特异性及眼针疗法对腹泻型肠易激综合征(D-IBS)的作用机制。方法:SPF级雄性Wistar大鼠40只,采用慢性应激结合束缚法建立D-IBS大鼠模型,随机分为正常组、模型组、穴区组和非穴区组。后3组先造模,穴区组针刺眼针"下焦区""大肠区""肝区""脾区";非穴区组选取同名眼针穴区外3mm处。模型组与正常组不予任何干预。测定各组大鼠粪便含水率;RT-PCR法检测各组大鼠结肠组织水通道蛋白8(AQP 8)的mRNA表达;SABC免疫组化法检测各组大鼠结肠组织血管活性肠肽(VIP)和AQP 8的蛋白表达。结果:与正常组比较,18d及25d时模型组、穴区组和非穴区组大鼠粪便含水率均明显增加(均P<0.01),结肠组织黏膜层、肌间神经丛和黏膜下神经丛VIP的蛋白表达水平均显著升高(均P<0.01),结肠AQP 8的mRNA表达水平显著降低(P<0.05,P<0.01),而模型组和非穴区组结肠黏膜层AQP 8蛋白表达明显减少(均P<0.01);与模型组比较,25d时穴区组大鼠粪便含水率明显减少(P<0.01),结肠组织VIP蛋白表达水平明显降低(P<0.05),结肠AQP 8的mRNA及蛋白表达水平明显升高(P<0.05,P<0.01);与穴区组比较,25d时非穴区组大鼠粪便含水率明显升高(P<0.01),VIP蛋白表达水平显著升高(均P<0.01),结肠AQP 8的mRNA及蛋白表达显著降低(均P<0.01)。结论:针刺眼针穴区能够有效治疗D-IBS,其可能的作用机制之一与上调结肠组织中AQP8并同时抑制VIP的表达有关。而针刺眼周非穴区部位则无明显疗效,从而说明眼针穴区部位具有相对特异性。To explore the point specificity of eye-acupuncture and the mechanism of eye-acupuncture on diarrhea-predominant irritable bowel syndrome （D-IBS）. Methods Forty male Wistar rats of SPF grade were randomly divided into a normal group, a model group, a eye-acupuncture point （AA） group and a non-point （NA） group. The D-IBS rat model was established with the combination methods of the chronic stress and binding limbs. The AA group was treated by acupuncture at ＂low energizer area＂, ＂large intestine area＂, ＂liver area＂ and ＂spleen area＂, and the NA group by acupuncture at 3 mm apart from the same points area mentioned above, and the nor real group and the model group with no intervention. The rate of feces moisture content was detected. Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the mRNA of aquaporin 8 （AQP 8） in co Ion. Protein expressions of the vasoaetive intestinal peptide （VIP） and AQP 8 in colon were detected by SABC im- munohistochemistry method. Results Compared with normal group, the rate of feces moisture content at the 18th and 25th days, VIP protein in colon mucosa, myenteric nerve plexus and hypo-mucosa nerve plexus increased sig- nificantly （all P-（0.01 ）, and AQP 8 mRNA in colon mucosa decreased significantly in model, AA and NA group （P〈0.05, P〈i0.01） ; AQP 8 protein in colon mucosa decreased significantly in model group and NA group （both P〈0.01）. Compared with model group, the rate of feces moisture content at the 25th day and VIP protein in colon mucosa decreased significantly （P〈0.01, P（0.05）, and AQP 8 mRNA and protein increased significantly（P＇~O. （）5,P^O. 01） in AA group, Compared with AA group, the rate of feces ncloisture coDlent al the 25th day and VIP protein in colon mucosa increased significantly （both P^0.01）, and AQP 8 mRNA and protein decreased significantly （both P^0.01） in HA group. Conclusion Eye acupuncture has a good therapeutic effect on 1） lt？~S. It is sugges

关 键 词：腹泻型肠易激综合征 眼针 水通道蛋白8 血管活性肠肽 

分 类 号：R245.3[医药卫生—针灸推拿学]