兔肝VX2肿瘤模型MSCT灌注成像与血管生成的关系研究  

Study of the Correlation Between MSCT Perfusion Imaging and Angiogenesis in Rabbit VX2 Liver Tumor Model

作　　者：曹新山[1] 秦东京[1] 姜兴岳[1] 张林[1] 刘泉源[1] 

机构地区：[1]滨州医学院附属医院放射科,滨州256603

出　　处：《滨州医学院学报》2012年第4期252-254,257,共4页Journal of Binzhou Medical University

基　　金：山东省自然科学基金(Y2008C177);山东省科技发展计划(2010GSF10265)

摘　　要：目的研究兔肝VX2肿瘤多层螺旋CT(MSCT)增强表现,以及其与肿瘤微血管密度(MVD)、血管内皮生长因子(VEGF)之间的变化关系。方法选取25只实验兔,分为对照组和实验组,采用MSCT灌注成像和免疫组化方法对对照组、实验组、兔肝VX2肿瘤模型组于建模后的7、14、21和28 d进行检查,测定分析MSCT灌注参数值:肝动脉灌注量(HAP)、门静脉灌注量(HPP)、肝动脉灌注指数(HPI)以及VEGF和MVD表达的变化,并分析MSCT灌注参数值和VEGF、MVD表达的相关性。结果实验组的HAP、HPI、VEGF和MVD值显著高于对照组,HPP明显低于对照组,差异均有统计学意义(P<0.05),实验组兔肝VX2模型组的HAP、HPP和HPI值以及其VEGF和MVD值在模型建立后不同时间段的差异均有统计学意义(P<0.05),HAP的变化与VEGF、MVD的变化具有显著正相关性(P<0.05),HPP、HPI的变化与VEGF、MVD变化无明显相关性(P>0.05)。结论 MSCT灌注成像可以提供肝动脉和门静脉灌注的量化信息,HAP与MVD、VEGF表达有明显正相关性,可间接反映肝肿瘤的血管生成。Objective To investigate the enhancement features of Multi-Slice Sprial CT（MSCT） and its correlation with microvessel density（MVD） and vascular endothelial growth factor（VEGF） in rabbit VX2 liver tumor model.Methods 25 rabbit VX2 liver tumor models were divided into control group and experiment group.MSCT perfusion scan and immunnohistochemical techniques were performed on those rabbits at 7,14,21 and 28 d after haven established with VX2 liver tumor successfully.The value of MSCT perfusion parameters： hepatic arterial perfusion（HAP）,hepatic portal perfusion（HPP） and hepatic perfusion index（HPI）,the expression of VEGF and MVD were determined and analyzed,and the correlations between them were analyzed.Results The value of HAP,HPI,VEGF and MVD in experiment group were much higher than those in control group,while the value of HPP in experiment group was much lower（P0.05）.The value of all the index above were all significantly different in different time after the VX2 liver tumor model had established successfully（P0.05）.There was significant positive correlation between MVD,VEGF and HAP（P0.05）,while no correlation was observed between HPP,HPI and MVD,VEGF（P0.05）.Conclusion MSCT perfusion can give a quantitative assessment of blood perfusion of hepatic artery and portal vein.HAP had significant positive correlation with MVD and VEGF,and it can reflect the liver tumor angiogenesis indirectly.

关 键 词：VX2肝肿瘤模型 MSCT灌注成像 血管生成 

分 类 号：R814.42[医药卫生—影像医学与核医学]