橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析  

Identification and analysis of proteins in the latex C-serum related to the tapping panel dryness in Hevea brasiliensis

作　　者：周雪梅[1,2] 杨礼富[1] 王真辉[1] 邹智[1] 袁坤[1] 

机构地区：[1]中国热带农业科学院橡胶研究所,农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737 [2]海南大学农学院,海南海口570100

出　　处：《南京林业大学学报（自然科学版）》2012年第5期37-41,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：中央级公益性科研院所基本科研业务费专项(YWFZX2008-12,2009-12,2010-9);海南省重点科技项目(90107);现代农业产业技术体系建设专项资金(CARS-34-02A)

摘　　要：橡胶树死皮是影响天然橡胶产量的重要限制因子。通过双向凝胶电泳技术(2-DE)研究了橡胶死皮树与健康树胶乳C-乳清蛋白表达谱的差异。以软件分析后共获得31个差异表达的蛋白点,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)分析后,搜索NCBInr数据库,结果有10个蛋白点被成功鉴定,其中6个为未知蛋白,其余4个蛋白分别为chain A,struc-ture of ubiquitin-like protein,Rub1;β-1,3-glucanase;AIR9 protein和flagellar inner dynein arm heav-y chain 11。这些蛋白可能在橡胶树死皮发生过程中扮演重要角色。The tapping panel dryness（TPD） in Hevea brasiliensis is a significant limit factor influencing natural rubber production.In this experiment,the two-dimensional gel electrophoresis（2-DE） was used to study the differentiation of protein expression profiles of latex C-serum between TPD trees and healthy ones of H.brasiliensis.Thirty-one differentially expressed protein spots were obtained by software analysis.After these spots were subjected to matrix assisted laser desorption/ionization time of flight mass spectrometry（MALDI-TOF MS） followed by searching for NCBInr database,ten spots were successfully identified.Six of them were identified as the unknown proteins and the remaining 4 were the chain A,structure of ubiquitin-like protein,Rub1;β-1,3-glucanase;AIR9 protein and flagellar inner dynein arm heavy chain 11.These proteins might play important roles in the process of TPD occuring.

关 键 词：橡胶树死皮 胶乳C-乳清 蛋白鉴定 双向凝胶电泳 MALDI-TOFMS 

分 类 号：S794.1[农业科学—林木遗传育种] Q503[农业科学—林学]