引发对马尾松种子细胞周期和发芽率的影响  

Effects of priming on cell cycle and germination of Pinus massoniana seeds

作　　者：任淑娟[1] 喻方圆[1] 徐丽萍[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2012年第5期51-55,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省普通高校研究生科研创新计划资助项目(苏教研〔2009〕8号)

摘　　要：采用流式细胞术研究不同引发处理下马尾松种子胚细胞DNA含量的差异,测定不同引发处理下马尾松种子的发芽率。结果表明:(1)PEG 8000溶液引发处理能促进胚细胞DNA的复制,4倍体细胞的比例增加,其中以20%PEG 8000溶液引发3～4 d的处理对种子胚细胞DNA合成的促进作用最明显。(2)胚细胞增殖指数最高的引发处理是20%PEG 8000溶液在15℃时引发3～4 d。(3)较高浓度的PEG 8000溶液和较长的引发时间有利于提高马尾松种子发芽率。(4)在一定范围内,胚细胞增殖指数与种子发芽率呈显著正相关。In order to reveal the cytological mechanism of promoting seed germination through seed priming,the differences of DNA content in embryonic cell of Pinus massoniana seeds after different priming treatments were studied by using flow cytometry,and seed germination percentage was also determined.The results showed that：（1） DNA replication in embryonic cell could be enhanced by PEG 8000 solution pretreatment,the ratio of tetraploid cells increased,the best treatment was a regime treated for 3-4 days using 20 % PEG 8000 solution.（2） The best priming treatment for increasing cell proliferation index was a regime treated in 20 % PEG 8000 solution for 3-4 days at 15 ℃.（3） Higher PEG 8000 concentration and longer pretreatment time were benefit for increasing germination percentage.（4） In a certain extent,there was a significant positive correlation between the cell proliferation index and seed germination percentage.

关 键 词：马尾松种子 引发处理 细胞周期 种子萌发 PEG8000 流式细胞术 

分 类 号：S722[农业科学—林木遗传育种]