免疫相关性全血细胞减少患者树突细胞表面分子CD80、CD86的表达及其临床意义  

Expression of CD80 and CD86 on dendritic cells of patients with immune related pancytopenia and its clinical significance

作　　者：滕广帅[1] 付蓉[1] 刘惠[1] 王红蕾[1] 王一浩[1] 阮二宝[1] 瞿文[1] 梁勇[1] 王国锦[1] 王晓明[1] 刘鸿[1] 吴玉红[1] 宋嘉[1] 王化泉[1] 邢莉民[1] 关晶[1] 王珺[1] 李丽娟[1] 邵宗鸿[1] 

机构地区：[1]天津医科大学总医院血液肿瘤科,300052

出　　处：《中华血液学杂志》2012年第10期865-868,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金（30971286、30971285）;高等学校博士学科点专项科研基金（200800620004）;天津市自然科学基金（08JcYBJC07800、09JCYBJC11200）;卫生部卫生行业科研专项（201002024）;天津医科大学重点学科基金（2009xk50、2009xk51）;天津市卫生局科技基金（210KZ105）;天津医科大学基金（2010ky20）

摘　　要：目的研究免疫相关性全血细胞减少（IRP）患者树突细胞（DC）的功能，探讨DC在IRP发病中的作用。方法应用流式细胞术检测65例IRP患者及17名健康对照者外周血髓系树突细胞（mDC，Lin—HLA—DR＋CD11c＋细胞）、浆细胞样树突细胞（pDC，Lin-HLA—DR＋CD123＋细胞）表面共刺激分子CD80、CD86的表达水平。结果IRP初治组（37例）、恢复组（28例）pDC表面CD86表达分别为（82．47±13．17）％、（60．08±14．29）％，均明显高于正常对照组[（47．95±18．59）％]（P值均〈0．05），初治组明显高于恢复组（P〈0．05）。初治组pDC表面CD80表达[（6．31±4．49）％]明显高于恢复组[（3．09±2．93）％]和正常对照组[（2．33±2．25）％]（P值均〈0．05）。初治组mDC表面CD86表达[（97．06±4．82）％]明显高于正常对照组[（91．35±12．20）％]（P〈0．05）。初治组、恢复组mDC表面CD80表达分别为（6．20±5．44）％、（3．97±3．24）％，均明显高于正常对照组[（1．86±1．73）％]（P值均〈0．05）。IRP组pDC表面CD86表达水平与Th1／Th2呈负相关（r=-0．733，P〈0．05），与骨髓单个核细胞膜表面抗体阳性率及CD5＋B细胞数量均呈正相关（r分别为0．283、0．436，P值均〈0．05），与血红蛋白、血小板、白细胞水平均呈负相关（r分别为-0．539、-0．519、-0．567，P值均〈0．05）；CD80表达水平与血红蛋白、血小板呈负相关（r分别为-0．431、0．464，P值均〈0．05）。结论IRP患者外周血pDC功能增强，可能与IRP发病有关。Objective To investigate the function of dendritic cells （DC） of patients with immune related pancytopenia （IRP） and explore the role of DC in IRP. Methods The expression of CDS0 and CD86 on myeloid DC （mDC, Lin-HLA-DR ＋ CD11c＋ cells） and plasmacytoid DC （pDC, Lin-HLA-DR ＋ CD123 ＋ cells） of 65 IRP （37 untreated and 28 remitted） patients and 17 healthy controls were analyzed by flow cytometry. Results The expression of CD86 on pDC was （82. 47±13. 17 ）% in untreated group and （60.08 ±14.29）% in remission group, which were significantly higher than that of controls （47.95 ± 18.59 ） % （ P 〈 0.05 ） , while the expression in untreated group was higher than that of remission group （ P 〈 0.05）. The expression of CD80 on pDC was （6.31 ± 4.49）% in untreated group, which was significantly higher than that of remitted patients （3.09 ± 2.93 ） % and controls （2.33 ± 2.25 ） % （P 〈 0.05）. The expression of CD86 on mDC was （97.06 ±4.82）% in untreated group and （91.35 ±12.20）% in control group, while the expression in untreated group was higher than that of control group （ P 〈 0.05 ）. The expression of CD80 on mDC was （6.20 ± 5.44 ） % in untreated group and （ 3.97± 3.24 ） % in remission group, which were significantly higher than that of controls （ 1.86 ± 1.73 ） % （ P 〈 0.05 ）. The expression of CD86 on pDC was negatively correlated to Th1/Th2 （r = -0. 733,P 〈 0.05 ） ,it was positively correlated to the an- tibody on membrane of BMMNC （ r = 0. 283, P 〈 0.05 ） and the quantity of CD5 , B eells （ r = 0. 436, P 〈 0.05 ） , while it was negatively eorrelated to the level of hemoglobin, platelets and white blood eells （r = -0. 539 ,P 〈0.05 ; r = -0. 519 ,P 〈0.05 ; r = -0.567 ,P 〈0.05, respeetively）. The expression of CD80 on pDC was negatively correlated to the level of hemoglobin and platelets （ r = - 0. 431, P 〈 0.05 ; r = - 0. 464, P 〈 0.05 ）. Conclusion The funetion of pDC in PB of IRP

关 键 词：全血细胞减少 自身免疫疾病 树突细胞 

分 类 号：R559[医药卫生—血液循环系统疾病]