1-溴丙烷经口染毒致大鼠周围神经病模型的建立  

Development of peripheral neuropathy rat model induced by 1-bromopropane

作　　者：王清华[1] 钟志霞[1] 陈晶晶[1] 谢克勤[1] 赵秀兰[1] 

机构地区：[1]山东大学公共卫生学院毒理学研究所,济南250012

出　　处：《中华劳动卫生职业病杂志》2012年第10期751-755,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金（81172708）;山东省科技攻关计划课题（2011GSF11814）

摘　　要：目的观察1-溴丙烷（1-bromopropane，1-BP）的周围神经毒性，建立1-BP经口染毒致周围神经病的动物模型。方法40只雄性Wistar大鼠，随机分为低、中、高剂量组和对照组，每组10只；将1-BP溶于玉米油中，分别经口给予200、400、800mg／kg的1-BP，对照组给予等体积玉米油，灌胃体积均为0．2ml／100g，每日1次，每周5d，连续16周。定期观察各组大鼠步态，测定后肢抓力和后肢撑力，染毒8周和16周时检测血液学和生化指标。结果高剂量组染毒8～16周、中剂量组染毒14～16周和低剂量组染毒15、16周大鼠的步态评分均明显高于对照组，差异有统计学意义（P〈0．05，P〈0．01）。与对照组比较，高剂量组染毒9、12、14周大鼠后肢抓力明显下降，差异有统计学意义（P〈0．05，P〈0．01）；染毒16周时，动物后肢瘫痪。染毒16周时，中、低剂量组大鼠后肢抓力分别为对照组的72．6％和91．2％，差异有统计学意义（P〈0．01，P〈0．05）。染毒12、14、16周时，高剂量组大鼠后肢撑力指数明显高于对照组；染毒14、16周时，中剂量组大鼠后肢撑力指数明显高于对照组，差异均有统计学意义（P〈0．05，P〈0．01）。染毒8周高、中剂量组和染毒16周高、中、低剂量组血清中丙氨酸转氨酶（ALT）活力均明显低于对照组，差异均有统计学意义（P〈0．01）。结论神经系统是1-BP毒作用的敏感靶器官，1-BP暴露可导致大鼠周围神经病。Objective To observe the peripheral neurotoxicity of 1-bromopropane （1-BP） by developing an animal model of peripheral neuropathy through oral administration of 1-BP. Methods Forty male Wistar rats were randomly and equally divided into low-dose group （200 mg/kg）, medium-dose group （400 mg/kg）, high-dose group （800 mg/kg）, and control group. The rats in the low-dose, medium-dose, and high-dose groups were orally given 1-BP （dissolved in corn oil）, while the rats in the control group were orally given an equal volume of corn oil. The oral administration （0.2 ml/100 g BW） was performed once per day, 5 days per week, for 16 consecutive weeks. Neurobehavioral indices including gait score, hindlimb grip strength, and hindlimb landing foot splay were recorded periodically. Hematological and biochemical parameters were also measured during and after 1-BP exposure. Results The gait scores were significantly higher in the high- dose group （after 8-16 weeks of 1-BP exposure）, medium-dose group （after 14-16 weeks of 1-BP exposure）, and low-dose group （after 15-16 weeks of 1-BP exposure） than in the control group （P〈0.05, P〈0.01 ）. Compared with the control group, the high-dose group showed significantly decreased hindlimb grip strength after 9, 12, and 14 weeks of 1-BP exposure （P〈0.05, P〈0.01 ）, with the hindlimbs paralyzed after 16 weeks of 1-BP exposure. After 16 weeks of 1-BP exposure, the hindlimb grip strengths of rats in the medium-dose and low-dose groups were decreased to 72.6% and 91.2% of the control value （P〈0.01, P〈0.05）. Compared with the control group, the high-dose group showed significantly increased hindlimb landing foot splay after 12, 14, and 16 weeks of 1- BP exposure, and the medium-dose group showed significantly increased hindlimb landing foot splay after 14 and 16 weeks of 1-BP exposure （P〈0.05, P〈0.01 ）. The high-dose and medium-dose groups showed significantly higher serum alanine aminotransferase （ALT） activity than the contr

关 键 词：1-溴丙烷 步态评分 后肢抓力 后肢撑力 周围神经病 

分 类 号：R114[医药卫生—卫生毒理学]