非O157产志贺毒素大肠杆菌分离株亚碲酸钾抗性水平及抗性基因簇分析  

Analysis of tellurite resistance level and resistance gene cluster in non-O157 Shiga toxin-producing Escherichia coli isolates

作　　者：白向宁[1] 赵爱兰[1] 孟琼[1] 徐建国[1] 熊衍文[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206

出　　处：《中华微生物学和免疫学杂志》2012年第7期585-588,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的了解我国部分地区非0157产志贺毒素大肠杆菌（Shigatoxi-producingEschen-richiacoli，STEC）分离株的亚碲酸钾抗性水平、抗性基因簇及其关系。方法使用平皿法检测亚碲酸钾抗性水平，采用PCR方法检测亚碲酸钾抗性基因簇。结果在所检测的39株非0157STEC中，仅有5株菌亚碲酸钾抗性水平介于128～512μg／ml，同时携带亚碲酸钾抗性基因簇（terABCDE）。另有2株菌的亚碲酸钾抗性水平为8μg／ml，3株菌为2μg／ml，其余29株菌均〈1μg／ml，且这34株菌ter-ABCDE均阴性。结论大多数非0157STEC分离株对亚碲酸钾敏感，在使用含亚碲酸钾的选择性培养基分离非0157STEC时应慎重。Objective To investigate the tellurite resistance level, the presence of tellurite resist- ance （ter） gene cluster and their relationships in non-O157 Shiga toxin-producing Escherichia coli （STEC） isolates. Methods Tellurite resistance level was evaluated by plate dilution method and the ter gene cluster was tested by PCR. Results Only 5 of 39 non-O157 STEC isolates tested in this study were identified to have ter gene cluster, which showed relatively high levels of tellurite resistance ranging from 128 μg/ml to 512 μg/ml. In contrast, the other 34 isolates without ter gene cluster were sensitive to potassium tellurite and showed very low levels of tellurite resistance, the minimal inhibitory concentration （MIC） was 〈 1 μg/ml for 29 isolates, 8 μg/ml for 2 isolates and 2μg/ml for 3 isolates. Conclusion Most non-O157 STEC iso- lates were sensitive to potassium tellurite. It could be concluded that much attention should be paid when screening the non-O157 STEC isolates using the selective medium supplemented with potassium tellurite.

关 键 词：非O157产志贺毒素大肠杆菌 亚碲酸钾抗性 ter基因簇 

分 类 号：R378[医药卫生—病原生物学]