小鼠胫骨中段1/3不同缺损直径单层骨皮质缺损模型比较研究  

A COMPARATIVE STUDY ON EFFECT OF DIFFERENT DEFECT DIAMETERS ON HEALING IN MIDDLE 1/3 TIBIA MONOLAYER CORTICAL BONE DEFECT MOUSE MODEL

作　　者：李福兵[1] 徐永清[1] 潘兴华[2] 李霞[1] 刘华[1] 杨杜明[1] 李军[1] 沙勇[1] 石健[1] 赵万秋[1] 

机构地区：[1]成都军区昆明总医院,骨科全军创伤骨科研究所,昆明650032 [2]成都军区昆明总医院,干细胞与组织器官工程中心,昆明650032

出　　处：《中国修复重建外科杂志》2012年第10期1218-1222,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金面上项目(81171734);中国博士后科学基金面上项目(20100481529);云南省应用基础研究项目(2011FZ312);昆明总医院院管课题(2009Y050C)~~

摘　　要：目的比较研究不同缺损直径对小鼠胫骨中段1/3单层骨皮质缺损模型愈合的影响,为组织工程材料研究、骨缺损修复及其分子机制研究和骨缺损基因治疗研究等提供动物模型。方法取8周龄雄性C57BL/6J小鼠10只,体重(20±2)g,随机分为A、B两组,每组5只。利用牙科磨钻分别制备直径为0.8 mm(A组)和1.0 mm(B组)小鼠胫骨中段1/3单层骨皮质缺损模型。于造模后7、21、28 d摄钼靶X线片观察缺损修复情况;28 d对骨缺损修复行Micro CT扫描及骨组织三维成像;28 d取材行HE染色观察。结果 B组5只小鼠造模7 d内均发生二次骨折,A组无骨折发生。X线片、Micro CT和HE染色均显示A组胫骨单层骨皮质缺损可在28 d达骨性愈合。Micro CT定量分析骨小梁示,A组骨小梁数目、骨小梁密度、骨体积显著高于B组,骨质密度显著低于B组,差异均有统计学意义(P<0.05);两组骨小梁分离度、骨小梁厚度差异无统计学意义(P>0.05)。结论小鼠胫骨中段1/3单层直径0.8 mm骨皮质缺损模型是研究胫骨缺损无外固定缺损修复机制和骨替代植入材料的理想动物模型。Objective To compare the effect of different defect diameters on healing in the middle 1/3 tibia monolayer cortical bone defect mouse model so as to establish an animal model for bone tissue engineering study, mechanism study on bone defect repair, and gene therapy research. Methods Ten 8-week-old C57BL/6J mice, weighing （20 ＋ 2） g, were randomly divided into 2 groups, 5 mice in each group. The middle 1/3 tibiae monolayer cortical bone defect model of 0.8 mm （group A） or 1.0 mm （group B） in diameter was established with burr drill. At 7, 21, and 28 days after modeling, the molybdenum target X-ray radiography was used to observe the defect repair; at 28 days, Micro CT and three-dimensional imaging were used to evaluate bone defect repair, and tibia specimens were harvested for HE staining. Results At 7 days after modeling, tibia fracture occurred in 5 mice in group B, no fracture in group A. X-ray films, Micro CT scan, and HE staining showed bony union in group A at 28 days. The quantitative analysis of trabecular bone by Micro CT showed that trabecular number, connectivity density, and bone volume in group A were significantly greater than those in group B （P 〈 0.05）, mean of segmented region--mean 2 was significantly less than that in group B （P 〈 0.05）, but no significant difference was found in trabecular separation and trabecular thickness between 2 groups （P 〉 0.05）. Conclusion The middle 1/3 tibia monolayer cortical bone defect mouse model of 0.8 mm in diameter is the ideal animal model for study repair mechanism of tibia defect or bone tissue engineering.

关 键 词：胫骨 骨缺损 动物模型 小鼠 

分 类 号：R687.3[医药卫生—骨科学]