脂肪源性干细胞临床转化应用中的相关问题  

RELATED ISSUES IN CLINICAL TRANSLATIONAL APPLICATION OF ADIPOSE-DERIVED STEM CELLS

作　　者：刘宏伟[1] 程飚[2] 付小兵[3] 

机构地区：[1]暨南大学附属第一医院整形外科再生医学教育部重点实验室,广州510630 [2]广州军区广州总医院整形外科全军热区损伤救治与组织修复重点实验室 [3]解放军总医院第一附属医院全军创伤修复重点实验室

出　　处：《中国修复重建外科杂志》2012年第10期1242-1246,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家重点基础研究发展计划资助项目(2005CB522603);国家自然科学基金资助项目(30772257;30973127;81171812);中央高校基本科研业务费专项资金资助项目(21611416)~~

摘　　要：目的对近年来国内外有关脂肪源性干细胞(adipose-derived stem cells,ASCs)在临床转化应用中应该或必须关注的问题进行综述。方法广泛查阅近年相关文献,对ASCs生产和使用过程中有关产品的管理、生产、运输、使用、安全性等方面进行综合分析。结果 ASCs作为成体干细胞家族的新成员在再生医学领域展现了广阔的应用前景。目前在美国国立卫生研究院(NIH)的http://www.clinicaltrials.gov网站上已注册登记的在15个国家进行的临床试验达40多个,说明世界范围内从事干细胞研究和应用的学者对ASCs临床转化应用的浓厚兴趣和重视。在临床转化应用中,ASCs产品存在管理,生产应遵循的质量控制标准,病原微生物污染的预防措施,分离过程中对酶和相关试剂的要求,取材过程中供区、年龄和性别可能的影响,低温贮藏,产品运输以及安全性等一系列问题。结论 ASCs作为成体干细胞具有较好的临床转化应用优点,在临床应用中对其存在的问题必须给予高度重视和进一步研究,以加速其临床转化过程。Objective To introduce the related issues in the clinical translational flapplication of adipose-derived stem cells（ASCs）.Methods The latest pflapers were extensively reviewed,concerning the issues of ASCs production,management,transportation,use,and safety during clinical flapplication.Results ASCs,as a new member of adult stem cells family,bring to wide flapplication prospect in the eld of regenerative medicine.Over 40 clinical trials using ASCs conducted in 15 countries have been registered on the website（http：//www.clinicaltrials.gov） of the National Institutes of Health（NIH）,suggesting that ASCs represents a promising flapproach to future cell-based therflapies.In the clinical translational flapplication,the related issues included the quality control standard that management and production should follow,the prevention measures of pathogenic microorganism pollution,the requirements of enzymes and related reagent in separation process,possible effect of donor site,age,and sex in sampling,low temperature storage,product transportation,and safety.Conclusion ASCs have the advantage of clinical translational flapplication,much attention should be paid to these issues in clinical flapplication to accelerate the clinical translation process.

关 键 词：脂肪源性干细胞 基质血管成分细胞 转化医学 再生医学 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]