大鼠急性β射线皮肤损伤创面愈合过程中Ang-1及其受体的表达  

Expressions of angiopoietin 1 and its receptor in acute β irradiated skin injury in rats

作　　者：沈国良[1] 杨明忠[1] 陆兴安[1] 王修珍[2] 

机构地区：[1]苏州大学附属第一医院烧伤整形外科,江苏省215006 [2]苏州大学医学部基础医学与生物科学学院

出　　处：《江苏医药》2012年第17期2010-2012,共3页Jiangsu Medical Journal

基　　金：苏州市科技发展计划(应用基础研究)基金资助项目(SYS201019)

摘　　要：目的探讨急性β射线皮肤损伤创面难愈合过程中血管生成素1(Ang-1)及其受体Tie-2表达的动态变化。方法雌性大鼠54只分为3组:照射组(n=24),直线加速器产生β射线(45Gy)单次照射大鼠臀部皮肤40mm×40mm,建立急性β射线皮肤损伤模型;烧伤组(n=24),以直径为25mm的恒温电烙铁,温度80℃,时间8s,建立大鼠深Ⅱ度热力烫伤动物模型;对照组(n=6),正常大鼠。取不同时期创面皮肤组织,采用免疫组化SP法及RT-PCR等方法,检测1、2、3、4周不同时相点各组大鼠创面组织中Ang-1蛋白、Tie-2蛋白和Ang-1mRNA、Tie-2mRNA的表达。结果正常大鼠皮肤中Ang-1、Tie-2积分吸光度(A)值低表达;照射组伤后1、2和3周的Ang-1 A值分别为0.130±0.012、0.170±0.013和0.192±0.072,明显低于烧伤组的0.190±0.182、0.332±0.014和0.273±0.014(P<0.05);照射组1、2和3周的Tie-2A值分别为0.112±0.011、0.183±0.012和0.171±0.061,亦明显低于烧伤组的0.170±0.062、0.310±0.013和0.284±0.014(P<0.05);Ang-1mRNA、Tie-2mRNA表达的变化与Ang-1蛋白、Tie-2蛋白相似。结论急性β射线皮肤损伤创面愈合过程中,Ang-1及Tie-2持续低表达可能是急性β射线皮肤损伤创面难愈合的机制之一。Objective To investigate the dynamic expressions of angiopoietin 1 （Ang-1）and its receptor Tie-2 in acute B-ray-irradiated skin injury. Methods Fifty-four female SD rats were divided into 3 groups. The models of acute β-ray-irradiated skin injury were established by radiation with 45 Gy βray on the skin in 24 rats（group A）. The models of deep second degree burn were established by electrical scald instrument with constant temperature 80℃, 8 s and 25 mm as common trattmatogenic factors in 24 rats （group B）. Six normal rats were served as normal controls （group C）. The expressions of Ang-1 and Tie-2 proteins and Ang-1 mRNA and Tie-2 mRNA were semi-quantitatively checked by immunohistochemical SP technique and RT-PCR, respectively at 1,2 and 3 weeks after radiation. Results Lower expressions of Ang-1 and Tie-2 proteins were identified in rat skin in group C. The Ang-1 absorbance values of 1,2 and 3 weeks in group A were 0. 130±0. 012,0. 1704±0. 013 and 0. 192±0. 072,which were lower than O. 190±0. 182,0. 332±0. 014 and 0. 273±0. 014 in group B（P〈0. 05）. The Tie-2 absorbance values of 1,2 and 3 weeks in group A were 0. 1124±0. 011,0. 183 ±0. 012 and 0. 171±0. 061,which were lower than 0. 170±0. 062,0. 310±0. 013 and 0. 284!0. 014 in group B（P〈0. 05）. The changes of Ang-1 mRNA and Tie-2 mRNA were similar to those of Ang-1 and Tie-2 proteins. Conclusion The persisting lower expressions of Ang-1 and Tie-2 may be one of the mechanisms involved in the of retarded healing of acute β-ray radiation injured skin wounds.

关 键 词：Β射线 放射性皮肤损伤 血管生成素1 受体TIE-2 

分 类 号：R818.895[医药卫生—放射医学]