百草枯对蜜蜂寿命及抗氧化酶基因表达影响  

Effects of paraquat on lifespan and level of expression of antioxidant enzyme genes in worker bees of Apis mellifera ligustica

作　　者：王文祥[1] 王欢[1] 张飞[1] 吴小波[1] 曾志将[1] 

机构地区：[1]江西农业大学蜜蜂研究所,南昌330045

出　　处：《应用昆虫学报》2012年第5期1155-1159,共5页Chinese Journal of Applied Entomology

基　　金：国家蜂产业技术体系资助项目(CARS-45-kxj12)

摘　　要：本研究用200、400和600mg/L的百草枯饲喂刚出房的意大利蜜蜂Apis mellifera ligustica Spinola工蜂,检测其对工蜂寿命及抗氧化酶基因Sod 1和Sod 2表达的影响,并以饲喂蔗糖作为对照组。结果表明:随着百草枯浓度的增加,工蜂的寿命极显著下降(P<0.01);抗氧化酶基因Sod 1的相对表达量,随着百草枯浓度的增加而增加,其中对照组Sod 1基因的相对表达量显著低于各百草枯处理组(P<0.05);Sod 2基因的相对表达量,随着百草枯浓度的增加,先增加后减少,其中600 mg/L百草枯处理组Sod 2基因的相对表达量显著低于对照组和低剂量百草枯处理组(P<0.05)。We investigated the effects of paraquat on lifespan and the level of expression of mRNA for the antioxidant enzyme genes Sod 1 and Sod 2 in newly emerged worker bees of Apis mellifera ligustica Spinola. Paraquat was given at doses of 200 rag/L, 400 mg/L and 600 mg/L. Bees in the control group were fed sucrose. The results showed that lifespans of honey bees decreased with the increase of paraquat concentration（ P 〈 O. 01 ）. The relative level of expression of the antioxidant enzyme gene Sod I increased linearly with the increase of paraquat concentration and it was significantly lower in the control group than in the paraquat-treated groups （ P 〈 0. 05）. In contrast, the relative level of expression of the antioxidant enzyme gene Sod 2 increased initially but then decreased with the increase of paraquat concentration. The level of expression of this gene was significantly lower in the high-dosage paraquat-treated group （600 mg/L） than that in both the control group and the low-and middle-dosage paraquat-treated groups （P 〈 0.05）.

关 键 词：意大利蜜蜂 工蜂 寿命 抗氧化酶基因 

分 类 号：S891[农业科学—特种经济动物饲养] S482.3[农业科学—畜牧兽医]