三叶蔓荆子提取物对小菜蛾的拒食活性  

Antifeeding activity of Vitex trifolia extracts against third-instar larvae of Plutella xylostella

作　　者：高燕[1] 盛广为[2] 沈嘉祥[2] 肖春[2] 

机构地区：[1]广东省农业科学院植物保护研究所,广州510640 [2]云南农业大学植物保护学院,昆明650201

出　　处：《应用昆虫学报》2012年第5期1298-1303,共6页Chinese Journal of Applied Entomology

基　　金：国家国际科技合作项目(2011DFB30040);广东省科技计划项目(2011B031500020)

摘　　要：室内测定了三叶蔓荆子(Vitex trifolia(L.))叶片提取物对小菜蛾Plutella xylostella(L.)3龄幼虫的拒食活性。非选择性试验结果表明,三叶蔓荆子的不同溶剂(乙酸乙酯、石油醚、氯仿和乙醇)提取物对小菜蛾均有一定的拒食作用。4种提取物对小菜蛾的毒力顺序为:乙酸乙酯提取物>石油醚提取物>氯仿提取物>乙醇提取物。选择性试验表明,乙酸乙酯提取物对小菜蛾24、48h的拒食中浓度(AFC50)分别为2 520、3240mg.L-1。室内盆栽试验结果表明,施用乙酸乙酯提取物(10000mg.L-1)3d后甘蓝(Brassica oleracea var.capitata)植株上3龄小菜蛾的虫口减退率可达72.76%。The antifeeding activity of extracts of Vitex trifolia （L.） leaves against the 3rd instar larvae of Plutella xylostella （L.） was tested in a laboratory. A no-choice experiment revealed that extracts extracted with either ethyl acetate, petroleum, ether or chloroform had significant antifeeding activity. The relative antifeeding activity of extracts obtained with each of these four solvents was, in descending order; ethyl acetate 〉 petroleum ether 〉 chloroform 〉 ethanol. A choice-experiment indicated that the AFCso of the ethyl acetate extract was 2 520 mg.L-1 24 h after treatment and 3 240 mg . L-1 after 48 h. The number of P. xylostella larvae on Brassica oleracea var. capitata plants could be reduced by 72.76% three days after spraying with ethyl acetate extract.

关 键 词：三叶蔓荆子 小菜蛾 拒食活性 

分 类 号：S482.3[农业科学—农药学]