5-FU乙醇脂质体制备及局部植入治疗家兔喉气管狭窄的效果分析  

Preparation of Ethosomes encapsulated with 5-fluorouracil and the effect of local administered 5-FU ethosomeon on laryngotracheal stenosis of rabbit

作　　者：杨希之[1] 敖华飞[1] 程雪峰[1] 顾健[1] 孔德秋[1] 毛小慧[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院耳鼻咽喉-头颈外科,上海201900

出　　处：《临床耳鼻咽喉头颈外科杂志》2012年第20期939-943,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：上海市卫生局青年基金项目资助(No:2010Y141);上海市教委优青基金项目资助(No:jdy10020)

摘　　要：目的:评价5-氟尿嘧啶(5-FU)脂质体对家兔瘢痕性喉气管狭窄的治疗效果。方法:利用薄膜分散法制备5-FU乙醇脂质体,并检测脂质体形态、包封率等特性。采用刮擦法制备喉气管狭窄模型,环形刮除气管黏膜约0.5cm宽度,诱导气管内瘢痕形成,将诱导瘢痕性喉气管狭窄成功的模型动物随机分为3组:5-FU乙醇脂质体组(A组)、5-FU水溶液组(B组)和生理盐水组(C组),各组分别在内镜监视下经皮穿刺将药物注射入瘢痕内部,并记录此时狭窄度,记为0d,其后,在用药后7、14、21d纤维喉镜下观察记录气管狭窄情况。结果:治疗初期A组和B组并无差别,21d时A组狭窄度明显小于B组,并在观察期间没有出现再狭窄。结论:5-FU脂质体对家兔瘢痕型喉气管狭窄的治疗具有较好的效果,可以作为喉气管狭窄的治疗方法进行进一步研究。To evaluate the efficacy of Ethosomes encapsulated with 5-FU in treatment of laryngotr acheal stenosis in rabbit models. Method：The 5-FU ethosome was prepared by the thin film hydration method, and the size distribution and the encapsulation efficiency was investigated. The tracheal mucosa was scraped about 0.5 cm in width with a nylon brush to induce the scar formation in the airway,then animals were divided into three groups：5-FU ethosome group,5-FU group and saline group. Drugs were injected into scar by paracentesis under endoscope in each group respectively. The severity of stenosis was observed under laryngofiberoseope immediately, 7,14,21 days after administration. Result：Airway stenosis of 5-FU ethosome group was not significantly different compared with 5-FU group at 7 days after administration, but 5-FU ethosome significantly reduced the airway ste- nosis at 21 days after administration when compared with 5-FU group and no restenosis was noticed during the ob servation period. Conclusion：Ethosomes encapsulated with 5-FU was effective for laryngotracheal stenosis. It is a ptentially new method for ameliorating airway stenosis originated from granulation tissue.

关 键 词：5-氟尿嘧啶 乙醇脂质体 气管狭窄 狭窄度 

分 类 号：R767.7[医药卫生—耳鼻咽喉科]