针刺“足三里”“太冲”穴对腹泻型肠易激综合征大鼠胃肠激素的影响  

Effect of Acupuncture at “Zusanli”(ST 36) and “Taichong”(LR 3) on Gastrointestinal Hormone Levels in Rats with Diarrhea Type Irritable Bowel Syndrome

作　　者：刘美荣[1] 肖瑞飞[2] 彭芝配[2] 左和宁[1] 朱克[2] 王思明[2] 

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]湖南中医药大学,长沙410007

出　　处：《针刺研究》2012年第5期363-368,共6页Acupuncture Research

基　　金：湖南省中医药管理局资助项目(No.2010016);湖南省科技厅资助项目(No.2011FJ 3245)

摘　　要：目的:观察针刺"足三里""太冲"对腹泻型肠易激综合征(IBS-D)大鼠胃肠激素的影响,为临床治疗IBS-D提供实验依据。方法:将48只健康Wistar大鼠随机分为空白组、模型组、针刺组、药物组,每组12只。采用慢性温和束缚应激加孤养法加灌服番泻叶,造成IBS-D大鼠模型。针刺组针刺大鼠双侧"足三里"穴和"太冲"穴,药物组每天灌胃匹维溴铵。采用双抗体夹心酶联免疫吸附测定法(ELISA)测定各组大鼠血浆和回肠生长抑素(somatostatin,SS)、P物质(substance P,SP)和血管活性肠肽(vasoactive intestinal peptide,VIP)的含量。结果:模型组与空白组相比,大鼠体质量显著降低(P<0.05),摄食量显著降低(P<0.01),腹泻指数显著升高(P<0.01);针刺组及药物组与模型组相比,大鼠体质量、摄食量、腹泻指数均明显改善(P<0.05,P<0.01)。模型组与空白组相比,大鼠血浆VIP含量显著升高(P<0.01),血浆SS和SP含量的差异无统计学意义(P>0.05);针刺组、药物组与模型组相比,大鼠血浆VIP含量显著降低(P<0.05)。模型组与空白组相比,大鼠回肠SS、VIP、SP含量均显著升高(P<0.01);针刺组、药物组与模型组相比,大鼠回肠SS、VIP、SP含量均显著降低(P<0.01)。结论:针刺"足三里""太冲"治疗IBS-D可能与抑制肠道SS、VIP、SP的分泌有关。Objective To observe the effect of acupuncture of ＂Zusanli＂ （ST 36） and ＂Taichong＂ （LR 3） on gastroin- testinal hormone levels in diarrhea type irritable bowel syndrome （IBS-D） rats so as to provide experimental evidence for acu- puncture treatment of IBS-D. Methods Forty-eight Wistar rats were randomly divided into control, model, acupuncture and medication groups, with 12 rats in each group. IBS-D model was established by chronic mild restraining stress combined with iso- lated raising and intragastric administration of Folium Sennae （10 mL/kg）. For rats of the acupuncture group, bilateral ＂Zusanli＂ （ST 36） and ＂Taichong＂ （LR 3） were punctured and stimulated for 30 min, once daily for 14 days. The rats of the medication group were treated by gavage of Pinaverin bromide, once daily for 14 days, and those of the control and model groups were given with equal volume of saline. Somatostatin （SS）, substance P（SP） and vasoactive intestinal peptide （ VlP ） in the rats＇ plasma and ileum tissues were detected by sandwich enzyme-linked immunosorbent assay （ELISA）. Results Compared with the control group, the body weight and food intake quantity were reduced significantly, while the diarrhea index was increased significantly in the model group （P〈0. 05, P〈0. 01 ）. In comparison with the model group, the body weight and food intake volume in both acu- puncture and medication groups were increased remarkably after the treatment （P〈0.05, P〈0. 01） whereas the diarrhea index in the latter two groups was reduced obviously （P〈0.01）. Compared with the control group, plasma VIP level was increased significantly in the model group （P〈0. 01 ）, while those in both acupuncture and medication groups were decreased remarkablycompared with the model group （P〈0.05）. No significant changes of plasma SP and SS levels were found after modeling and af- ter both acupuncture and medication treatments. In comparison with the control group, ileum SP, VIP a

关 键 词：针刺 腹泻型肠易激综合征 胃肠激素 

分 类 号：R245.311[医药卫生—针灸推拿学]