头针对心肌缺血再灌注大鼠心交感放电、心肌β_1-肾上腺素受体蛋白表达及血浆去甲肾上腺素含量的影响  

Effects of Electroacupuncture Stimulation of Scalp-point on Cardiac Sympathetic Discharges,Myocardial β_1-adrenoceptor Protein Expression and Plasma Norepinephrine Concentration in Myocardial Ischemia-reperfusion Injury Rats

作　　者：李美平[1] 孙国杰[1] 尤行宏[1] 

机构地区：[1]湖北中医药大学,武汉430065

出　　处：《针刺研究》2012年第5期385-389,共5页Acupuncture Research

摘　　要：目的:观察头针"额旁1线"对心肌缺血再灌注损伤大鼠交感神经活动、心肌β1-肾上腺素受体(β1-AR)蛋白表达及血浆去甲肾上腺素(NE)含量的影响,探讨头针防治心肌缺血再灌注损伤的作用机制。方法:Wistar大鼠随机分成假手术组、模型组、头针组,每组6只。采用结扎左冠状动脉前降支30min,再灌注15min制备大鼠心肌缺血再灌注模型。取头部双侧"额旁1线"区域进行电针治疗,频率14Hz,强度为2V。用BL-420E+生物信号采集系统记录左交感神经放电情况,通过免疫印迹法检测缺血区心肌β1-AR蛋白表达变化,利用酶联免疫吸附法测定血浆NE含量的变化。结果:模型组和头针组大鼠在缺血30min和再灌注15min后,交感神经放电频率较缺血前及假手术组明显加快(P<0.01,P<0.05),而头针组较模型组明显减慢(P<0.05)。模型组大鼠心肌β1-AR蛋白表达增加,血浆NE含量明显上升,与假手术组比较差异有统计学意义(P<0.01);头针组大鼠缺血区心肌β1-AR蛋白表达显著下调,血浆NE浓度明显降低,与模型组比较差异有统计学意义(P<0.05)。结论:头针可通过调整心肌缺血再灌注大鼠交感神经放电频率、心肌β1-AR蛋白表达水平及血浆NE含量,减轻钙超载,使紊乱的心肌细胞电活动趋于和缓,发挥其抗再灌注心肌损伤作用。Objective To explore the mechanism of electroacupuncture （EA） of scalp-point in the management of myo- cardial ischemia-reperfusion injury （MI/RI） by examining its effects on left cardiac sympathetic nerve activity, myocardial β1- adrenaline receptor （AR） protein expression and plasma norepinephrine （NE） concentration in MI/RI rats. Methods Eighteen Wistar rats were randomly divided into sham, model and EA groups （n = 6）. MI/RI model was induced by ligation of the left ante- rior descending coronary artery for 30 min, followed by release of the ligatien for 15 min. EA was applied to bilateral Epangxian I （MS 2） for 15 min. The left cardiac sympathetic nerve activity was recorded with BL-420 E＋ biological signal acquisition system, Myocardial β1-AR protein expression was examined by western blot and plasma NE level detected by enzyme-labeled immunosor- bent assay. Results Compared with the sham group, the left cardiac sympathetic discharges and plasma NE levels and myocar- dial β1-AR protein expression were markedly increased in the model group （P〈0. 01 ）, whereas in comparison with the model group, the sympathetic discharges, plasma NE level and myocardial β1-AR protein expression in the EA group were down-regula- ted significantly （P〈0.05）. Conclusion Scalp-point EA intervention can suppress MI/RI induced increase of sympathetic nerve activity and plasma NE level, andβ1-AR protein expression, which may contribute to its effect in relieving myocardial ischemia.

关 键 词：心肌缺血再灌注 电针 头穴 交感神经活动 心肌β1-肾上腺素受体蛋白表达 血浆去甲肾 上腺素含量 

分 类 号：R245.321[医药卫生—针灸推拿学]