羊膜上皮细胞胶原海绵复合体的构建及其细胞特性变化  

Construction of complex of rat amniotic epithelial cells and collagen sponge and its growth and cellular characteristics~

作　　者：何俊山[1] 许家军[1] 齐爱青[1] 郭兵[1] 曹文珞[1] 刘芳[1] 姬瑞娟[1] 王德伟[2] 

机构地区：[1]第二军医大学解剖学教研室,上海200433 [2]常州市第一人民医院烧伤整形科,常州213000

出　　处：《解剖学杂志》2012年第5期567-570,F0004,共5页Chinese Journal of Anatomy

基　　金：国家973重点基础研究发展计划（2005CB724302）;常州市卫生局重大科研项目（ZD200801）

摘　　要：目的：构建羊膜上皮细胞（AECs）胶原海绵复合体，并研究其细胞生长及特性。方法：将大鼠AECs接种于胶原海绵支架中，一般贴壁培养细胞作对照，用CM-Dil及Hoechest33342标记细胞膜和细胞核、HE染色、扫描电镜等观察细胞的生长和形态，CCK-8法检测细胞活力，免疫细胞化学、实时荧光定量PCR检测细胞表型。结果：与一般贴壁培养AECs相比，AECs在胶原海绵中粘附生长良好，增殖能力强，直径变小，细胞表型变化不明显，仅NestinmRNA表达上调。结论：胶原海绵支架与AECs生物相容性良好，能维持细胞原特性并促进其生长和增殖。Objective： To construct complex of amniotic epithelial ceils （AECs） and collagen sponge and to investigate its cel- lular characteristics. Methods： Rat AECs were seeded in the scaffold of collagen sponge and in the bottle for attachment cul- ture served as a control group. The growth and morphological changes were observed with CM-Dil labeling cell membrane, Hoechest33342 labeling nucleus, HE staining, scanning electron microscopy. Cellular viability was detected with CCK-8 kit. Cellular phenotype was examined by immunohistochemistry and real time quantitative PCR. Results： Compared with the culture in the bottle, rat AECs adhered and grew well within the scaffold, proliferated more actively. Its diameter de- creased. Its cellular phenotype did not change obviously, but nestin mRNA upregulated. Conclusion： Collagen sponge and AECs is proven to have a good biocompatibility, which could maintain previous cellular characteristics of AECs and promote AECs growth and proliferation.

关 键 词：羊膜上皮细胞 胶原海绵 生物相容性 细胞特性 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]