牛源地衣芽胞杆菌诱导小鼠临床乳房炎模型中热休克蛋白和核因子-κB表达上调  

Up-regulated expression of heat shock protein 70 and NF-gB in mouse mastitis model established by artificial infection of Bacillus licheniformis from bovine mastitis

作　　者：甘露[1] 徐君[1] 李姝[1] 程跃[1] 蔡亚非[1] 

机构地区：[1]安徽师范大学生命科学学院,生物环境与生态安全安徽省高校省级重点实验室,芜湖241000

出　　处：《解剖学杂志》2012年第5期596-599,共4页Chinese Journal of Anatomy

基　　金：“十二五”国家科技计划课题（2011BAD28802）;江苏省农业三项工程项目（SX2011-189）;安徽省科技人员服务企业计划项目（10020303043）;2011年度芜湖市科技计划项目;安徽省自然科学基金（1208085Mc41）

摘　　要：目的：为进一步认识奶牛乳腺炎发生的免疫病理机制和寻找更理想的防治方法提供基础研究数据。方法：从采集回来的奶牛奶样中用传统细菌培养方法和分子学方法分离鉴定主要病原菌，挑选其中地衣芽胞杆菌感染小鼠乳腺组织，H-E染色分析感染后小鼠乳腺组织变化，免疫组织化学方法分析热休克蛋白70（HSP70）和核因子-KB（NF-kB）在感染小鼠体内的表达。结果：感染后小鼠乳腺出现明显的炎症症状，组织病理学观察显示腺泡腔内有大量嗜中性粒细胞浸润。实验组中HSP70和NF-kB表达量比生理盐水组和空白组均有增加。结论：成功通过地衣芽胞杆菌诱发建立实验性乳房炎小鼠病理模型，实验组中HSP70和NF-kB表达量的增加说明其参与细菌感染小鼠乳腺组织后的病理过程。Objective： To further understand how the immune pathogenesis of cow mastitis occurs and look for better control methods to provide data for basic study. Methods.. In this study the main mastitis pathogens were isolated and identified by the traditional bacterial culture methods and molecular methods. Bacillus licheniformis was selected from the main pathogens. Then mouse mammary gland was infected by Bacillus licheniformis. HE staining was done to observe the change of the mouse mammary gland after infection and immunohistochemical staining to detect the expression of heat shock protein 70 （HSP70） and NF-kB in those slices. Results： Symptoms of inflammation appeared in the mouse mammary gland after infection. Histopathologic examination of mammary gland revealed that numbers of polymorphonuclear neutrophils were present in alveoli. The expression of HSP70 and NF-kB in the experimental group was significantly increased compared with the sa- line group and the blank group. Conclusion： The results showed that Bacillus licheniformis was suitable for the replica of mastiffs model in mouse. An increase in the expression of HSP70 and NF-kB in the experimental group explained their par- ticipation in the pathological process after bacterial infection.

关 键 词：奶牛 地衣芽胞杆菌 小鼠乳房炎模型 热休克蛋白70 核转录因子-KB 

分 类 号：R975[医药卫生—药品]