白藜芦醇衍生物白皮杉醇和乙酰化白藜芦醇辐射防护作用研究  

Radiation-protective effects of piceatannol and acetylated resveratrol

作　　者：王春燕[1] 郝述霞[1] 佟鹏[1] 齐雪松[1] 吕慧敏[1] 苟巧[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《癌变．畸变．突变》2012年第5期345-348,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:研究白藜芦醇(resveratrol)衍生物白皮杉醇(piceatannol)和乙酰化白藜芦醇(acetylated resveratrol)对受照射淋巴母细胞AHH-1和小鼠的辐射防护作用。方法:运用CCK-8试剂盒检测不同浓度(0、10、20、50、100和500μmol/L)白皮杉醇和乙酰化白藜芦醇作用72 h对AHH-1细胞的毒性作用,以及不同终浓度(0、10、20和50μmol/L)白皮杉醇和乙酰化白藜芦醇预处理AHH-1细胞2 h后对经4 Gy ^(60)Coγ射线照射的细胞存活率的影响。并进一步观察白藜芦醇、白皮杉醇和乙酰化白藜芦醇对^(60)Coγ射线辐射损伤模型小鼠外周血白细胞和受照后30 d内小鼠平均存活时间的影响。结果:与对照细胞(0μmol/L)相比,10、20和50μmol/L白皮杉醇和乙酰化白藜芦醇作用AHH-1细胞72 h对细胞存活无明显影响(P>0.05),但可显著提高受照AHH-1细胞的存活率(P<0.05或P<0.01)。受照后24 h,乙酰化白藜芦醇中(100 mg/kg)、高(200 mg/kg)剂量组小鼠外周血白细胞数与照射对照组相比显著升高(P<0.05)。与正常对照组比较,照射对照组小鼠外周血白细胞数和受照30 d内小鼠平均存活时间显著降低(P<0.05);与照射对照组相比,白藜芦醇和乙酰化白藜芦醇高(100 mg/kg)剂量组小鼠受照后30 d内平均存活时间明显延长(P<0.05)。结论:白藜芦醇衍生物白皮杉醇和乙酰化白藜芦醇均对受照射淋巴母细胞AHH-1细胞具有一定的辐射防护作用;乙酰化白藜芦醇对受照射小鼠亦具有显著的辐射防护作用。OBJECTIVE：To investigate radiation-protective effect of piceatannol and acetylated resveratrol. METHODS：CCK-8 Assay Kit was used to detect the survival rate of AHH-1 cells treated by 0,10,20,50,100 and 500μmol/L piceatannol or acetylated resveratrol for 72 h,or by 4 Gyγ-ray for 48 h after treatment with 0,10,20 and 50μmol/L piceatannol or acetylated resveratrol for 2 h.Kunming mice were treated with resveratrol,piceatannol and acetylated resveratrol before ^60Co γ-irradiation.The average life span and the WBC count post-irradiation were observed. RESULTS：Compared to control cells（0μmol/L）,the survival rate of AHH-1 cells treated by 10,20 and 50μmol/L piceatannol or acetylated resveratrol for 72 h was not changed（P0.05）,but the survival rate of post-irradiation AHH-1 cells treated by 10,20 and 50μmol/L piceatannol or acetylated resveratrol increased（P0.05 or,P0.01）.After radiation for 24 h,the WBC count of middle-dose（100 mg/kg） and high-dose（200 mg/kg） acetylated resveratrol groups increased（P0.05）. Compared to control group（0μmol/L）,the average life span and the WBC count of radiation group decreased（P0.05）. Compared to radiation group,the average life span of mice in high-dose resveratrol or acetylated resveratrol groups increased.CONCLUSION：Piceatannol and acetylated resveratrol both had radiation-protective effect in post-radiation AHH-1 cells.In addition,acetylated resveratrol showed radiation-protective effect in post-radiation Kunming mice.

关 键 词：白藜芦醇 白皮杉醇 乙酰化白藜芦醇 辐射 AHH-1 

分 类 号：R818[医药卫生—放射医学]