Genetic Transformation of Chinese Cabbage (Brassica rapa pekinensis) with Floral-dip Method  

作　　者：Yang GAO Xiangliang REN Songlin RUAN Huoxu WANG 

机构地区：[1]College of Life Science,Liaoning Normal University [2]Key Laboratory of Plant Biotechnology of Liaoning Province,Liaoning Normal University [3]Hangzhou Academy of Agricultural Sciences

出　　处：《Agricultural Biotechnology》2012年第4期22-24,27,共4页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of Liaoning Province (901113);a Horizontal Project of Hangzhou Academy of Agricultural Sciences

摘　　要：[ Objective] This study is aimed to transform gus gene into Chinese cabbage （Brassica rapa pekinensis） by floral-dip method. [ Method] The Chinese cabbage was transformed by floral-dip method for the first time. Then the cabbage seeds were harvested and screened by hygromycin, and the plants with resistance were confirmed by histochemical GUS assay and PCR detection. [ Result] The target gene was successfully integrated into the Chinese cabbage genome, and the transformation rate was 0.1%. [ Conclusion ] This study optimized the genetic transformation system for Chinese cabbage, and laid the foundation for improving the means of genetic transformation of Chinese cabbage.[ Objective] This study is aimed to transform gus gene into Chinese cabbage （Brassica rapa pekinensis） by floral-dip method. [ Method] The Chinese cabbage was transformed by floral-dip method for the first time. Then the cabbage seeds were harvested and screened by hygromycin, and the plants with resistance were confirmed by histochemical GUS assay and PCR detection. [ Result] The target gene was successfully integrated into the Chinese cabbage genome, and the transformation rate was 0.1%. [ Conclusion ] This study optimized the genetic transformation system for Chinese cabbage, and laid the foundation for improving the means of genetic transformation of Chinese cabbage.

关 键 词：Chinese cabbage AGROBACTERIUM Floral-dip TRANSFORMATION 

分 类 号：S634.1[农业科学—蔬菜学]