Effects of Sucrose on Total RNA Concentration during Formation of Microtubers of Pinellia ternata in vitro  

作　　者：Lan GUO Aimin ZHANG Jianping XUE Yanfang ZHU Wei SHENG 

机构地区：[1]School of Life Science,Huaibei Normal University/Anhui Key Laboratory of Plant Resources and Biology

出　　处：《Agricultural Biotechnology》2012年第4期28-30,共3页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China (30973963);Anhui Provincial Natural Science Foundation (090413252);Key Program of Natural Science Research of Anhui Provincial Universities (KJ2009A160)

摘　　要：[ Objective] This study aimed to investigate the variation rule of total RNA concentration during formation of microtubers of PineUia ternata induced by sucrose. [ Method ] Plantlets of P. ternata were used as experimental materials. Total RNA concentration was determined with spectrophotometer, and the variation of total RNA was analyzed before and after formation of P. ternata microtubers treated with different concentrations of sucrose using DDRT-PCR technology. [ Result] Total RNA concentration of P. ternata treated with different concentrations of sucrose varied greatly, which showed an upward trend in the first 15 d, de- clined from 15~ d to 25th d, and slightly raised from 25＇h d to 30＇8 d. Microtubers formed under various sucrose treatments showed an increase in RNA concentration after induction, and it was assumed that the treatment of high concentrations of sucrose might have activated the expression of development-related＇ genes in P. ter- nata microtubers. [ Conclusion] This study provides the basis for revealing the related gene expression information and molecular mechanism during formation of P. ternata microtubers induced by sucrose.[ Objective] This study aimed to investigate the variation rule of total RNA concentration during formation of microtubers of PineUia ternata induced by sucrose. [ Method ] Plantlets of P. ternata were used as experimental materials. Total RNA concentration was determined with spectrophotometer, and the variation of total RNA was analyzed before and after formation of P. ternata microtubers treated with different concentrations of sucrose using DDRT-PCR technology. [ Result] Total RNA concentration of P. ternata treated with different concentrations of sucrose varied greatly, which showed an upward trend in the first 15 d, de- clined from 15~ d to 25th d, and slightly raised from 25＇h d to 30＇8 d. Microtubers formed under various sucrose treatments showed an increase in RNA concentration after induction, and it was assumed that the treatment of high concentrations of sucrose might have activated the expression of development-related＇ genes in P. ter- nata microtubers. [ Conclusion] This study provides the basis for revealing the related gene expression information and molecular mechanism during formation of P. ternata microtubers induced by sucrose.

关 键 词：PineUia ternata MICROTUBER SUCROSE Total RNA DDRT-PCR 

分 类 号：S567.239[农业科学—中草药栽培]