梅州地区土种猪氟烷基因发生率的PCR-RFLP检测  

Detection on the Halothane gene in Meizhou pigs by PCR-RFLP

作　　者：韩春艳[1] 章庆民[1] 李庆昌[1] 刘奇伟[1] 洪静虹[1] 

机构地区：[1]广东嘉应学院生命科学学院,广东梅州514011

出　　处：《广东农业科学》2012年第18期163-164,169,共3页Guangdong Agricultural Sciences

摘　　要：选取71头梅州地区土种猪,从猪毛毛囊中提取基因组DNA,纯化后进行PCR-RFLP扩增,得到氟烷基因(RYR1)特异片段,经Hha I内切酶消化后,发现Hhan/Hhan基因型频率为2.8%,HhaN/HhaN基因型频率为94.4%,HhaN/Hhan基因型频率为2.8%。说明梅州地区土种猪氟烷敏感基因频率和基因型频率相对较高。Halothane was amplified by PCR from genomic DNA of 71 randomly-selected Meizhou pigs and found that Hhan/Hhan, HhaN/HhaN and HhaN/Hhan gene frequencies were detected to be 2.8%, 94.4% and 2.8% respectively. The results showed that Halothane gene frequencies in Meizhou were higher than other regions.

关 键 词：梅州土种猪 氟烷基因 基因频率 

分 类 号：S828[农业科学—畜牧学]