注射型rhBMP-2/PLGA/FS缓释微球载体系统用于兔桡骨骨缺损修复的实验研究  

Experimental Study on Application Recombinant Human Bone Morphogenetic Protein 2(rhBMP-2)/Poly-Lactide-co-Glycolic Acid(PLGA) / Fibrin Sealant(FS) on Repair of Rabbit Radial Bone Defect

作　　者：范仲凯[1] 曹阳[1] 张哲 张明超[1] 卢伟[1] 唐磊[1] 姚琦[3] 吕刚[1] 

机构地区：[1]辽宁医学院附属第一医院骨科,锦州121000 [2]锦州市第二医院骨科,锦州121000 [3]北京世纪坛医院骨科,北京100038

出　　处：《生物医学工程学杂志》2012年第5期903-907,共5页Journal of Biomedical Engineering

基　　金：辽宁省教育厅科学技术项目资助(L2010327);辽宁医学院第一临床学院青年科技基金资助项目(2010C16)

摘　　要：探讨注射型重组人骨形态发生蛋白-2/聚乳酸-羟基乙酸/纤维蛋白胶(rhBMP-2/PLGA/FS)缓释微球载体系统在修复兔桡骨节段性骨缺损中的作用。制作新西兰大白兔桡骨缺损模型,将其分为实验组(rhBMP-2/PLGA/FS),对照组(FS)和空白组(不作处理)。在缺损局部注入各组材料后,通过X射线观察各组动物的骨缺损修复情况;取兔桡骨损伤节段定量分析其骨密度值,评估其成骨质量;利用HE及Masson染色,观察骨组织形态及骨再生情况,评估其修复作用。结果表明:rhBMP-2/PLGA/FS缓释微球载体组在骨缺损修复、成骨质量及成骨能力方面均显著优于对照组和空白组,能够显著促进兔骨缺损修复,是一种较为理想、高效的骨组织工程材料。This paper is aimed to investigate the repair of rabbit radial bone defect by the recombinant human bone morphogenetie protein 2/polylactidecoglycolic acid microsphere with fibrin sealant （rhBMP2/PLGA/FS）. The ra dial bone defect models were prepared using New Zealand white rabbits, which were randomly divided into 3 groups, experiment group which were injected with eMP2/PLGA/FS at bone defect location, control group which were in jected with FS at bone defect location, and blank control group without treatment. The ability of repairing bone de feet was evaluated with Xray radiograph. Bone mineral density in the defect regions was analysed using the level of ossification. The osteogenetic ability of repairing bone defect, the degradation of the material, the morphologic change and the bone formation were assessed by HE staining and Masson staining. The result showed that rhBMP 2/PLGA/FS had overwhelming superiority in the osteogenetic ability and quality of bone defect over the control group, and it could promote the repair of bone defect and could especially repair the radial bone defect of rabbit well. It may be a promising and efficient synthetic bone ~raft.

关 键 词：重组人骨形态发生蛋白-2 缓释微球 纤维蛋白胶 骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]