雾化吸入变应原对支气管哮喘外周血CD4+CD25+Foxp3+Treg的影响  

Effects of Desensitization of Allergen Nebulization on Blood CD4+CD25+Foxp3+Treg in the Prevention and Treatment of Asthma

作　　者：李红华[1] 赵娟[2] 张景鸿[3] 

机构地区：[1]湖北医药学院附属太和医院病理科,湖北省十堰市442002 [2]湖北医药学院附属太和医院呼吸内科,湖北省十堰市442002 [3]广西医科大学研究生学院,广西南宁市530021

出　　处：《中华全科医学》2012年第11期1725-1727,共3页Chinese Journal of General Practice

摘　　要：目的探讨CD4+CD25+Foxp3+Treg细胞在哮喘变应原雾化吸入减敏治疗的作用。方法粉尘螨和屋尘螨点刺阳性的支气管哮喘患者随机分为A组(常规治疗)和B组(变应原吸入减敏),各20例。B组雾化吸入特异性变应原,A组雾化吸入以生理盐水代替,两组给予相同的基础治疗。治疗前、后用流式细胞术(FCM)检测外周血中CD4+CD25+Foxp3+T细胞占CD4+T细胞比例;进行哮喘控制评分和肺功能、气道反应性测定。结果治疗后B组ACT评分高于A组,两组肺功能均有明显增加。B组支气管激发试验转阴率明显高于A组。B组外周血中CD4+CD25+Foxp3+T细胞占CD4+T细胞比例较A组明显升高(P<0.05)。结论 CD4+CD25+Foxp3+T细胞在变应原雾化吸入减敏防治支气管哮喘中发挥免疫调节作用。Objective To evaluate the effect of allergen nebulization on the ratio of blood CD4 ＋ CD25 ＋ Foxp3 ＋ Treg in asth- ma. Methods 40 patients with Der. p and Der. f allergy and newly diagnosed uncontrolled moderate persistent bronchi＇a/asthma were randomly divided into 2 groups： group A and group B（20 per group）. The patients in group B were nebulized with specific allergen twice a week for 6 months. Both groups were treated with salmeterol xinafoate and flutieasone propionate powder. The per- centage of CD4 ＋ CD25 ＋ Foxp3 ＋ Treg cells in peripheral blood was determined by flow cytometry. ACT, airway responsiveness and lung function were performed before and after treatment. Results The negative conversion rates of Bronchial Provocation Test in group B were higher than group A significantly. The percentage of CD4 ＋ CD25 ＋ Foxp3 ＋ Treg cells increased in group B when compared with group A （ P 〈 O. 05 ）. Conclusion CD4 ＋ CD25 ＋ Foxp3 ＋ T ceils played an important immunomudulatory role in immunotherapy of allergen nebulization in treatment of asthma.

关 键 词：支气管哮喘 特异性免疫治疗 雾化 TREG 

分 类 号：R562.25[医药卫生—呼吸系统] R593.1[医药卫生—内科学]