PCR-DGGE技术鉴定高含盐生物脱硫反应器中的优势菌种  

Identification of the Superiority Strains in the Biological Desulfurization Reactor by PCR-DGGE Melhodology Treating High Salinity Wastewater

作　　者：王桂萍[1] 杨金生[1,2] 刘卫国[2,3] 梁存珍[2] 

机构地区：[1]沈阳理工大学材料科学与工程学院,辽宁沈阳110159 [2]北京石油化工学院环境工程系,北京102617 [3]武汉理工大学资源与环境工程学院,湖北武汉430070

出　　处：《中国沼气》2012年第5期10-13,共4页China Biogas

基　　金：北京科技新星计划资助项目(2008B25);环境治理与调控技术北京市优秀教学团队资助项目(PHR201107213)

摘　　要：为鉴定高含盐废水生物脱硫反应器中的优势菌种,从连续运行一年的生物脱硫反应器中获取微生物样品,用试剂盒提取微生物总DNA。使用细菌通用引物(GC341F/534R)从总DNA中成功扩增出16S rDNA片段,扩增的16S rDNA进行DGGE分离,对DGGE条带进行切胶和测序。测得的16S rDNA序列在GenBank数据库进行检索和同源性分析。结果表明,系统中有4种优势菌株:硫杆菌属的Thiobacillus sp.ISA11,盐生硫杆菌属的Halothiobacil-lus sp.ST15,Marine bacterium HB-5,Uncultured bacterium clone J69-151C-2A。其中,Thiobacillus sp.ISA11和Halothio-bacillus sp.ST15是主要的脱硫细菌。In order to identify the superiority strains in the biological desulfurization reactor treating high salinity wastewater,total microbial DNA was extracted from sludge samples of bio-desulfurization bioreactor operated continuously for 1 year.16S rRNA fragments were amplified with the universal primers（GC341F/534R）,and amplified DNA fragments were separated by denaturing gradient gel electrophoresis（DGGE）.The DGGE bands in the gel were excided for sequencing.Homology analysis was conducted by comparing these gene sequences based on GenBank database.Results showed that the superiority strains were Thiobacillus sp.ISA11,Halothiobacillus sp.ST15,Marine bacterium HB-5,Uncultured bacterium clone J69-151C-2A,among which Thiobacillus sp.ISA11 and Halothiobacillus sp.ST15 were the major desulfurizing strains.

关 键 词：生物脱硫 变性梯度凝胶电泳技术 无色硫细菌 高盐度含硫废水 

分 类 号：X703[环境科学与工程—环境工程]