五节芒离体再生与多倍体诱导技术体系的建立  

Establishment of an in vitro system for regeneration and polyploid induction of Miscanthus floridulus

作　　者：周玥玥[1] 陈智勇[1] 黄丽芳[2] 邓果特[1] 蒋建雄[1] 易自力[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《湖南农业大学学报（自然科学版）》2012年第5期487-490,F0002,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金："十二.五"农村领域国家科技计划项目(2011AA100209-3)

摘　　要：以五节芒(Miscanthus floridulus)幼穗为外植体,建立了五节芒的离体再生体系:幼穗的最佳愈伤诱导培养基为MS+4.0 mg/L 2,4–D+0.1 mg/L 6–BA,最佳分化培养基为MS+2.0 mg/L 6–BA,最佳生根培养基为MS+1.0mg/L IAA+0.1 mg/L IBA+1.0 mg/L CCC。比较不同浓度秋水仙碱溶液对五节芒愈伤组织诱变的效果,结果显示,以1 000 mg/L秋水仙碱处理愈伤组织72 h,诱导率可达12.48%,形态特征、DNA含量和染色体核型分析表明,诱导获得的再生植株为五节芒四倍体。The immature inflorescence of Miscanthus floridulus were used as explants to establish the regeneration system in vitro.The results indicate that the best medium for callus induction was MS medium with 4.0 mg/L 2,4–D,0.1 mg/L 6–BA,for bud differentiation was MS medium with 2.0 mg/L 6–BA and for plantlets rooting was MS medium with1.0 mg/L IAA,0.1 mg/L IBA and 1.0 mg/L CCC.The effects of different concentrations of colchicine on polyploid induction were investigated in vitro.The results indicate that the induction rate of polyploidy in the treatment with 1 000 mg/L colchicine solution for 72 h reached 12.48%.The morphological characteristic,nuclear DNA contents and chromosome karyotype of the regenerated plantlets showed the inducted plantlets were tetraploid.

关 键 词：五节芒 离体再生 秋水仙碱 多倍体 

分 类 号：Q943[生物学—植物学] Q949.94