水稻Pi2/9位点3个抗瘟基因的抗菌谱及稻瘟病菌遗传多样性分析  

Analysis of the antimicrobial spectrum of three rice blast resistance genes at Pi2/9 locus and genetic diversity of rice blast strains

作　　者：江南[1,2] 王素华[1,2] 李智强[1,2] 文婷[1,2] 梁毅[1,2] 吴俊[3] 杨婷婷[1,2] 戴良英[2,4] 王国梁[1,2] 刘雄伦[1,2] 

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]湖南农业大学生物安全科学技术学院,湖南长沙410128 [3]国家杂交水稻工程技术研究中心,湖南长沙410125 [4]作物基因工程湖南省重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2012年第5期506-510,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31171526)

摘　　要：采用35个不同来源的稻瘟病菌菌株,对3个稻瘟病抗性基因Pi9、Pi2、Pizt的供体亲本和以日本晴为遗传背景的转基因近等基因系进行室内接种鉴定,比较分析3个基因的抗谱差异。结果表明:Pi9和Pi2的抗谱较广,抗性频率均达到94.2%,Pi9对菌株ROR1和X2007A–7表现感病,Pi2对菌株CHNOS60–2–3和RB14表现感病,Pizt抗谱较窄,抗性频率仅为60%,使用13对SSR引物分析其中20个稻瘟病菌菌株的遗传多样性,可划分为4个宗谱,菌株的遗传宗谱和来源相关性不显著,与致病性有一定的相关性。In this study,35 blast strains,rice lines including the donors of blast resistant genes and rice transgenic lines with the genetic background of Nipponbare were employed for antimicrobial spectrum analysis of three blast resistant genes Pi9,Pi2 and Pizt.The results showed that Pi9 and Pi2 both have a broad spectrum of resistance with a resistance frequency of 94.2%,while Pizt have a narrow spectrum of resistance with a resistance frequency of only 60%.The blast strains ROR1,X2007A–7 are compatible to Pi9,and CHNOS60–2–3,RB14 are compatible to Pi2.In addition,13 pairs of SSR markers were used for analysis of the genetic diversity of 20 out of the 35 blast strains,the results showed these 20 strains were classified into 4 lineages which showed certain relationship with pathogenicities but no significant correlation with the origin of the strains.

关 键 词：水稻 稻瘟病菌 抗性基因 抗菌谱 病原菌遗传多样性 

分 类 号：S435.111.1[农业科学—农业昆虫与害虫防治] S425.111.41[农业科学—植物保护]