电针百会和长强穴对荷乳腺癌小鼠SCN部分输出信号分子水平的影响  

Influence of Electroacupuncture Changqiang(GV1)and Baihui(GV20) on SCN Output Signal Molecular of Mice Bearing Breast Cancer

作　　者：李胜吾[1] 薛红[1] 王俊娟[1] 刘文[1] 王子静[1] 吴佳[1] 张承舜[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川成都610075

出　　处：《南京中医药大学学报》2012年第5期457-460,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(30873297)

摘　　要：目的观察荷乳腺癌小鼠电针后其视交叉上核(suprachiasmatic nucleus,SCN)及外周血清和肿瘤组织中TGFα和受体EGFR水平的变化,探讨电针对荷瘤动物自发性活动节律影响的输出信号分子调控机制。方法将18只C57BL/6J雄性小鼠随机分为空白组、模型组、电针组3组,每组6只。LD驯化10d后关闭光源使动物处于自由运行状态,DD驯化10d后开始造模,造模成功继续监测节律7d后进行电针干预。电针组在CT4针刺百会和长强穴,使用韩氏穴位神经刺激仪,频率2/15Hz,电流0.5mA,每次15min,每日1次,连续电针3次。空白组和模型组在相应时相点CT4用同样的方法抓取和固定。第三次电针后2h立即断颈处死动物,在相应部位取材后使用酶联免疫吸附法(ELISA)对指标进行检测。结果电针后各组小鼠SCN中TGFα水平比较无统计学差异;各组小鼠外周血清、瘤组织中TGFα水平分别比较,模型组均值均大于空白组,差异具有统计学意义(P<0.05);电针组均值均低于模型组,但差异无统计学意义。电针后各组小鼠SPZ中EGFR水平比较,模型组均值大于空白组,但差异无统计学意义;电针组均值小于模型组,其差异具有统计学意义(P<0.05);各组小鼠外周血清、瘤组织中EGFR水平分别比较,模型组均值均大于空白组,其差异具有统计学意义(P<0.05),电针组均值小于模型组,但差异无统计学意义。电针后各组小鼠SCN和外周血清中PK2水平分别比较,模型组均值均小于空白组,但差异无统计学意义;电针组均值均大于模型组,其差异具有统计学意义(P<0.05)。结论荷乳腺癌小鼠外周血清和肿瘤组织中TGFα及EGFR水平较正常小鼠明显增高。电针可以明显降低荷乳腺癌小鼠SPZ中EGFR水平,增高SCN中枢和外周血清中PK2水平,提示电针干预可能通过直接调控中枢和外周PK2水平以及对SPZ中EGFR水平的直接调控间接影响TGFα水平从而影响荷瘤小鼠自发性活动节律。�OBJECTIVE Observe the change of TGFαand EGFR level in SCN,serum and tumor tissues of mice bearing breast cancer after elecreoacunpuncture for further discuss on the regulating mechanism of SCN output signal molecular of electroacupuncture on the spontaneous activity rhythm of animals bearing tumors.METHODS 18 male C57BL/6J mice were randomly divided into 3 groups with 6 in each group.LD domestication for 10 days,model establishment after DD domestication for 10 days and electroacupuncture after 7 days since model establishment succeed.Electroacupuncture on Changqiang（GV 1） and Baihui（GV 20） on CT4 with HANS Acupoint and Nerves Stimulator（HANS-200）,2/15 Hz,0.5 mA,15 min for each time.Electroacupuncture for three times with one time everday.Animals were sacrificed after 2 hours in the third time.Extract the sample and detect the indexes by ELISA.RESULTS There was no significant differences in SCN TGFαlevel among 3 groups;In the serum and tumor tissues,the TGFαlevels of model group were higher significantly than blank group（P0.05） while there was no significantly difference between electroacupuncture group and model group.There was no significantly difference in SPZ EGFR level between model group and blank group.The EGFR levels of electroacupuncture group were higher significantly than model group（P0.05）.In the serum and tumor tissues,the EGFR levels of model group were higher significantly than blank group（P0.05） while there was no significantly difference between electroacupuncture group and model group.In SCN and serum,the PK2 levels of electroacupuncture group were higher significantly than model group（P0.05） while there was no significantly difference between blank group and model group.CONCLUSION TGFα and EGFR levels in serum and tumor tissues of mice bearing breast cancer were significantly higher than normal mice.Electroacupuncture can decrease EGFR levels in SPZ and increase PK2 levels in SCN and serum of mice bearing breast cancer which suggested that electroacupuncture affe

关 键 词：电针 荷瘤 TGFΑ EGFR PK2 水平 影响 

分 类 号：R285[医药卫生—中药学]