一贯煎对CCl_4诱导肝纤维化大鼠肝细胞凋亡及其调控基因表达的影响  

Effect of "Yiguan Decoction" on Hepatocellular Apoptosis and Underlying Gene Expression in CCl_4-induced Hepatic Fibrosis

作　　者：曹健美[1,2] 陶庆[1] 慕永平[1] 冯琴[1] 彭景华[1] 刘平[1,2] 胡义扬[1] 

机构地区：[1]上海中医药大学附属曙光医院肝病研究所,上海201203 [2]上海中医药大学,上海201203

出　　处：《上海中医药大学学报》2012年第5期70-75,共6页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金重大研究计划重点项目(90409020);国家自然科学基金面上项目(30973924);上海市教委创新团队项目(第1期);国家中医药管理局中医肝胆病重点学科项目

摘　　要：目的:研究一贯煎对CCl4诱导的大鼠肝纤维化肝细胞凋亡及其调控基因表达的影响。方法:用50%CCl4橄榄油溶液(1 ml/kg)大鼠腹腔注射造模,每周2次,共9w。于造模3w和6w末,随机抽取正常及模型大鼠处死作动态观察。从第7w始模型大鼠随机分为模型对照组和药物干预组,药物干预组给予一贯煎灌胃,共计3w。9w末检测肝组织Fas、Bax、Bcl-xL、细胞色素(Cyt)C、Caspase-12、Caspase-3蛋白表达和肝细胞凋亡情况。结果:①与正常组大鼠比较,3w、6w及9w时模型大鼠肝组织Fas、Bax和Caspase-12蛋白表达逐渐升高(P<0.05,P<0.01)。与9w模型组比较,一贯煎组Fas、Bax和Caspase-12蛋白表达显著降低(P<0.01),而Cyt C和Bcl-xl蛋白表达在各组之间无显著差异(P>0.05)。②与正常组比较,造模3w时肝组织Caspase-3蛋白表达达到高峰(P<0.01),肝细胞凋亡数量亦达到高峰;6w、9w模型组大鼠Caspase-3蛋白表达和肝细胞凋亡较3w有降低的趋势。与9w模型组大鼠比较,一贯煎组大鼠Caspase-3蛋白表达显著降低,肝细胞凋亡显著减少。结论:一贯煎抑制CCl4诱导大鼠肝纤维化肝细胞凋亡的作用机制可能与干预Fas和内质网凋亡通路有关。Objective： To investigate the effects of ＂Yiguan Decoction＂ on hcpatocellular apoptosis and underlying gene expression regulation in CC14-induced hepatic fibrosis. Methods： Rats were treated with 50% CCI4 in oil （ip, 1 mg/kg, twice a week） for nine weeks to establish the model. Rats in control group and model group（ n =6 ） were sacrificed to observe its pathological condition dynamically after treatment for three weeks and six weeks. Rats were randomly divided into two groups： model group and ＂Yiguan Decoction＂ group which was treated with ＂Yiguan Decoction＂ for three weeks. After treatment, rats were sacrificed, and the protein expression of Fas, Bax, Bcl-xl, Cytochorome C （Cyto C）, Caspase-12 and Caspase-3 and hepatocellular apoptosis in fiver were measured. Results： （1）The protein expression of Fas, Bax and Caspase-12 in rats liver in model group increased gradually compared with control group after treatment for three weeks, six weeks, and nine weeks, respectively（ P 〈0.05, P 〈0.01 ）. After treatment for nine weeks, the protein expressions of Fas, Bax, and Caspase-12 in ＂Yignan Decoction＂ group were reduced significantly compared with model group （P 〈0.01 ）. However, there was no significant difference in the protein expression of Cyto C and Bcl-xl between the two groups. （2）The protein expression of Caspase-3 in liver reached a peak, coincident with the amount of hepatocellular apoptosis in model group after treatment with CC14 for three weeks. However, the protein expressions of Caspase-3 and hepatocellular apoptosis in model group tended to decrease after treatment with CC14 for six weeks and nine weeks. Besides, the protein expressions of Caspase-3 and hepatocellular apoptosis in ＂Yiguan Decoction＂ group reduced significantly compared with model group after treatment for nine weeks. Conclusion： ＂ Yiguan Decoction＂ can inhibit hepatic fibrosis and hepatocellulm＂ apoptosis in CC14-induced rat by reducing Fas expression and influencing end

关 键 词：一贯煎 肝纤维化 细胞凋亡 基因表达 

分 类 号：R285.5[医药卫生—中药学]