2株嗜碱性放线菌的分离鉴定及特性研究  

Isolation,identification and characterization of two basophilic actinomycosis

作　　者：熊鹰[1] 余金辉[1] 阿不都克里木·热依木[2] 魏艳红[1] 李逸[1] 莫小妍[1] 陈湖星[1] 袁永泽[1] 刘德立[1] 

机构地区：[1]华中师范大学生命科学学院遗传调控与整合生物学重点实验室,武汉430079 [2]新疆师范大学生命科学与化学学院,乌鲁木齐830054

出　　处：《华中师范大学学报（自然科学版）》2012年第5期596-600,605,共6页Journal of Central China Normal University：Natural Sciences

基　　金：湖北省科技攻关项目(2007AA301C26;2007AA201C50);教育部博士点基金项目(20060511002)

摘　　要：从新疆巴洲和硕县碱性土壤中分离纯化出2株放线菌菌株XJ-1和XJ-4,对其形态特征、生理生化特性、抗生素产生以及16S rRNA基因序列分析等方面进行了多种特性研究.结果表明.2株放线菌在pH7.0的中性条件下不能生长,在pH12.0的强碱性条件下能够生长,最适生长pH为10.0,属于嗜碱性放线菌.2株放线菌菌株能耐受10%的NaCl,最高耐受温度为45℃.菌株XJ-1产生抗生素,能抑制柑橘绿霉菌、水稻轮纹菌、棉花枯萎菌和小麦赤霉菌的生长.16S rRNA基因同源性分析表明XJ-1属于拟诺卡氏菌属的Nocardiopsis dassonvillei,序列相似性达到了99.9%.而XJ-4与Nocardiopsis. sp. AF-333的序列相似性达到了99.3%,有可能是一新种.Two actinomycete strains XJ-1 and XJ-4 were isolated from the alkaline soil ofShuo County and BaZhou County in Xinjiang Province. Based on the morphology, physiological and biochemical characteristics, antibiotics production and 16S rRNA genesequence analysis, the results showed that the optimum growth pH of these two basophilie actinomycosis was 10.0 ; they could not grow in the neutral conditions of pHT. 0 and could grow in the strong alkaline conditions of pill2.0. Two actinomycete strains had a tolerance of 10% NaC1 and the highest tolerance of temperature were 45℃. XJ-1 was able to produce antibiotics. It could obviously inhibit the growth of Penicillium digitatum, but also inhibit the growth of Rhizoetonia solani, Fusariumoxysporum and Fusarium raminearum. XJ-1 was preliminarily determined to be the Nocardiopsis dassonvillei, their sequence similarity reached 99.9%. The homology of the 16S rDNA sequences of strain XJ-4 with Nocardiopsis. sp. AF-333 was more than 99.0%, so the strain XJ-4 maybe a new species belonged to the basophilie aetinomycetes.

关 键 词：嗜碱性放线菌 分离 鉴定 抗生素 

分 类 号：Q93[生物学—微生物学]