产色素非O1群霍乱弧菌的发现与微生物学研究  

Discovery of Ten Strains of Non-O1 V. Cholerae Pigmentogens and Microbiological study

作　　者：丁业荣 贾利君[2] 时全 解少煜 赵本海 常宏伟 马振华 汪军 杨杰 

机构地区：[1]安徽省六安地区卫生防疫站,六安市237008 [2]中国药品生物制品检定所

出　　处：《中国卫生检验杂志》2000年第2期145-148,共4页Chinese Journal of Health Laboratory Technology

基　　金：安徽省自然科学资金资助

摘　　要：［目的］研究产色素非Ｏ１群霍乱弧菌在水源水中的分布、生物学性状及其致病性。［方法］在本地主要饮用水源设点、采样、培养致病性弧菌。分离、鉴定该菌过程中用营养琼脂斜面代替双糖铁琼脂取得良好效果。［结果］１９９４～１９９８年在水源水中相继检出１７株产色素的革兰氏阴性弧状菌，经形态学、培养特性、生化学、血清学的系统鉴定，均符合非Ｏ１群霍乱弧菌的定义，动物实验等显示其有效强毒力。［结论］因该菌在硷性胆盐琼脂、营养琼脂斜面和血琼脂上培育后能产生水溶性褐色色素，故命名为产色素非Ｏ１群霍乱弧菌。Objective]: To study the distribution of non-of Vibrio cholerae pigmentogens in water and its biological characteristics and pathogenicity. [Methods ]: Samples were collected from local source of drinking water and cultured for pathogenic V. cholerae. Non-of V. cholerae wae isolated and identified with nutrient agar slant instead of KIA. [Results ]: Seventeen strains of V. cholerae producing pigment with negative Gram' s stain were detected in the source water in Lu 'an Prefecture, Anhui Province during 1994 to 1998, which could all met the definition ofnon- 01 V. cholerae in morphology, cultural characteristics, biochemistry, with systematic identification, Animal experiments showed that it had strong virulence. [Conclusion ]: These strains of V. choleraecultured in basic cholate,nutrient agar slant and blood agar could produce brown pigment, so it was called non-of V. cholerae pigmentogens.

关 键 词：水污染 生物学性状 产色素非O1群霍乱弧菌 

分 类 号：R378.3[医药卫生—病原生物学]