甲醛滴定法测定牛血清白蛋白的酶解度  

Determination of Degree of Enzymatic Hydrolysis of BSA by Formol Titration Method

作　　者：王剑锋[1] 孙爽[1] 李瑶[1] 盖明昊[1] 肖龙[1] 张坤[1] 刘宝全[1] 范圣第[1] 

机构地区：[1]大连民族学院生物化学工程国家民委-教育部重点实验室,辽宁大连116605

出　　处：《大连民族学院学报》2012年第5期441-444,共4页Journal of Dalian Nationalities University

基　　金：教育部科学技术研究重点项目(2010-263);国家民委项目(09DL08);辽宁省教育厅资助项目(2009A154);大连民族学院人才启动基金资助项目(20096102)

摘　　要：针对作者前期提出的蛋白质酶解度定义,发展了一种利用甲醛滴定实验测定蛋白质酶解度的方法。分别利用甘氨酸与赖氨酸研究了α位游离氨基和ε位游离氨基在甲醛滴定实验中的测量状况,结果表明,甲醛滴定法测量结果比实际值偏低,进而利用修正系数对测定结果进行校正,确定甘氨酸、赖氨酸与牛血清白蛋白的修正系数分别为0.797,1.292和52.47,利用甲醛滴定法测定并计算出胰蛋白酶对底物牛血清白蛋白在本实验特定酶解条件下的酶解度为22.7。In this paper,the DEH（degree of enzymatic hydrolysis） of Bovine Serum Albumin（BSA） was tested and calculated with formol titration method.The conception of DEH was established and named and a determination method was developed based on formol titration.A correction coefficient was introduced to correct the determination results because of the weakness of formol titration.The values of correction coefficient of glycine and lysine in formol titration were 0.797 and 1.292,but it was 52.47 in BSA because of its abundant lateral chain groups.The α-amino group was different from ε-amino group in formol titration.According to the definition and the calculation formula of DEH,the enzymatic hydrolysis results of the BSA,which digested with trypsin in limited condition,were obtained by the formol titration method and the DEH was 22.7 in this research.

关 键 词：酶解度 甲醛滴定法 蛋白质 酶 水解 

分 类 号：Q519[生物学—生物化学]