活性氧与NO在SO_2诱导蚕豆气孔运动中的作用  

Involvement of ROS and NO in SO_2-Induced Stomatal Movement in Vicia faba Leaves

作　　者：仪慧兰[1] 刘鑫[1] 尹晶晶[1] 

机构地区：[1]山西大学生命科学学院,太原030006

出　　处：《河南大学学报（自然科学版）》2012年第5期593-598,共6页Journal of Henan University:Natural Science

基　　金：国家自然科学基金(30470318;30870454);山西省回国留学人员科研项目资金(2009022);高等学校博士学科点专项科研基金(20070108007)联合资助

摘　　要：以蚕豆叶表皮为材料,研究SO2胁迫时叶面气孔运动及其调节途径.研究发现,用浓度1～200μmol/L的SO2衍生物(亚硫酸钠与亚硫酸氢钠混合液)处理蚕豆叶下表皮后,气孔开度明显减小,气孔保卫细胞内活性氧(ROS)、一氧化氮(NO)和钙离子(Ca2+)水平显著升高.采用抗氧化剂抗坏血酸和过氧化氢酶,钙离子干扰剂EGTA和LaCl3,以及NO合成抑制剂NaN3与NO清除剂c-PTIO,分别与SO2衍生物同时作用时,SO2诱发的气孔关闭效应得到有效缓解,保卫细胞内ROS、NO和Ca2+水平随之改变.抗氧化剂和NO干扰剂能阻止SO2诱导的胞内ROS、NO和Ca2+水平升高;EGTA和LaCl3能降低SO2诱导的胞内NO和Ca2+升高,但不影响ROS水平.研究结果表明,较高浓度SO2能诱导气孔关闭,SO2胁迫诱导ROS和NO合成增加,ROS和NO通过钙信号系统调节气孔开度.Sulfur dioxide （SO2） is a common air pollutant. In order to understand how plants respond to environmental SO2, stomatal aperture as well as levels of intracellular reactive oxygen species （ROS）, nitric oxygen （NO） and Ca2＋ in guard cells were investigated in Vicia faba leaves in this study. The results of the present study showed that stomatal aperture significantly decreased after epidermal strips exposed to 1 to 200 μmol/L SO2 derivatives for 2 h, associated with obvious increases of intracellular ROS, NO and Ca2＋ levels in Vicia guard cells. Antioxidants ascorbic acid （AsA, 1 mmol/L） and catalase （CAT, 200 U/mL）, NO antagonists c-PTIO （200μmol/ L） and NaNa （100 μmol/L）, and Ca2＋ antagonists EGTA （1 retool/L） and LaCl3 （0.1 mmol/L） efficiently blocked SO2-induced stomatal closure, accompanying alterations of intracellular ROS, NO and Ca2＋ levels. AsA, CAT, c- PTIO and NaN3 significantly blocked SO2-evoked intracellular ROS, NO and Ca2＋ elevation. EGTA and LaCl3 also blocked SO2 induced NO production, but did not affect ROS production. The results indicated that SO2 induced stomatal movement via ROS and NO mediating Ca2＋ signaling, demonstrated that plant could adopt to environmentalSO2 by regulating ROS and NO production to control stomatal movement and stomatal aperture.

关 键 词：SO2 气孔开度 ROS NO CA2+ 蚕豆 

分 类 号：Q945.78[生物学—植物学]