IL-1β,IL-8在使用复方苦参洗剂治疗小鼠瘙痒症中的表达  

Expression of I L-1β and I L-8 During the Course of Appling Compound Kuh-seng Lotion to Mice with Pruritus

作　　者：孙洁慧[1] 赵景文[1] 左进[2] 

机构地区：[1]甘肃中医学院,甘肃兰州730030 [2]甘肃省中医院

出　　处：《西部中医药》2012年第10期18-19,共2页Western Journal of Traditional Chinese Medicine

摘　　要：目的:观察复方苦参洗剂对细胞因子白介素-1β(IL-1β)和白介素-8(IL-8)引起皮肤瘙痒的治疗作用,初步探讨复方苦参洗剂治疗皮肤瘙痒的作用机理。方法:采用磷酸组胺建立小鼠瘙痒模型,用即用型SABC法免疫组化染色后对各皮损部位的切片进行IL-1β、IL-8含量检测。结果:与空白组和基质组相比,复方苦参洗剂高、中、低各剂量组IL-1β、IL-8灰度值均降低,其差异有统计学意义(P<0.05);与炉甘石洗剂组相比,复方苦参洗剂高、中剂量组IL-1β、IL-8灰度值降低,其差异有统计学意义(P<0.05)。结论:复方苦参洗剂可明显抑制瘙痒模型小鼠皮肤组织中IL-1β和IL-8的含量,其改变程度与用药剂量有密切关系。Objective： To observe the function of compound kuh-seng lotion on cytokine（interleukin-1β and interleukin-8） which induced pruritus and initially explore the mechanism of compound kuh-seng lotion in treating pruritus.Method： Pruritic mice model was established with histamine phosphate.Contents of IL-1β and IL-8 in slices were detected with SABC method.Result： Compared with blank group and stroma group,grey levels of IL-1β and IL-8 lowered in high,middle and low dose groups of compound kuh-seng lotion,the differences suggested statistical meaning（P 〈0.05）;compared with calamine lotion,grey levels of IL-1β and IL-8 decreased in high and middle groups of compound kuh-seng lotion,the differences demonstrated statistical meaning（P 〈0.05）.Conclusion： Compound kuh-seng lotion can obviously inhibit the contents of IL-1β and IL-8 in skin tissue of pruritic mice,the degree is closely associated with the dose.

关 键 词：白介素-1Β 白介素-8 瘙痒 复方苦参洗剂 

分 类 号：R758.3[医药卫生—皮肤病学与性病学]