消肿止痛合剂对大鼠急性脊髓损伤后NOS及MMP-9表达的影响  

The Influence of XiaoZhong ZhiTong Mixture on Expressions of NOS and MMP-9 in Rats with Acute Spinal Injury

作　　者：邓强[1] 许伟[1] 李盛华[1] 陈志龙[1] 

机构地区：[1]甘肃省中医院,甘肃兰州730050

出　　处：《西部中医药》2012年第10期40-43,共4页Western Journal of Traditional Chinese Medicine

基　　金：甘肃省科技厅项目(编号096RJZA041)

摘　　要：目的:观察消肿止痛合剂对大鼠急性脊髓损伤后细胞凋亡率、一氧化氮合成酶(NOS)及基质金属蛋白酶-9(MMP-9)的影响,探讨消肿止痛合剂治疗急性脊髓损伤的作用机理。方法:40只SD大鼠随机分为4组:空白对照组(A)、空白模型组(B)、甲基强的松龙组(C)、消肿止痛合剂组(D)。A组不做任何处理,B组、C组、D组采用脊髓半横切法造模后,B组给予生理盐水灌胃;C组给予肌注甲基强的松龙(30 mg/kg);D组给予消肿止痛合剂,每日用量为成人等效剂量10倍,3次/d,术后第8日起,2次/d,剂量不变。造模后14天,TUNEL染色法检测细胞凋亡率,大鼠尾静脉采血,放免法检测NOS含量,免疫组化法测定MMP-9的表达。结果:D组动物脊髓损伤部位细胞凋亡率明显低于B组(P<0.01),NOS含量低于B型组(P<0.01),同时B组大鼠脊髓组织中MMP-9表达明显高于D剂组(P<0.01)。结论:消肿止痛合剂对脊髓损伤具有保护作用,可能是通过降低动物脊髓损伤部位NOS,维持TIMPs/MMP-9平衡,抑制MMP-9表达,从而调节细胞凋亡,保护脊髓功能。Objective： To explore mechanism of XiaoZhong ZhiTong mixture in the treatment of acute spinal cord injury by observing the effects of XiaoZhong ZhiTong mixture on apoptosis rate,NOS and MMP-9 of rats with acute spinal cord injury.Method： Forty rats were randomized into four groups： blank control group（A）,blank model group（B）,methylprednisolone group（C） and XiaoZhong ZhiTong mixture group（D）.A remained unhandled.B,C and D were handled with spinal cord hemisection method.B received physiological saline through intragastric administration;C were administered with intramuscular methylprednisolone（30mg/kg）;D were given with XiaoZhong ZhiTong mixture,daily dose were ten times of adult equivalent dosage,three times per day,then changed to twice each day at the eighth day after surgery in the same dose.By the 14th day after establishing the models,apoptosis rate was detected with TUNEL tincture,NOS content was tested with radioimmunity and expression of MMP-9 detected with immunohistochemistry after drawing blood from caudal vein of rat.Result： Apoptosis rate of D was remarkably lower than B（P〈0.01）,NOS content was lower than B（P〈0.01）,meanwhile expression of MMP-9 in B was higher than D significantly（P〈0.01）.Conclusion： XiaoZhong ZhiTong mixture could protect spinal cord,which might regulate apoptosis and protect function of spinal function by decreasing NOS of injured region,maintain the balance of TIMPs/MMP-9 and inhibit the expression of MMP-9.

关 键 词：急性脊髓损伤 消肿止痛合剂 细胞凋亡率 NOS MMP-9 

分 类 号：R651.21[医药卫生—外科学]