制备高效液相色谱法快速纯化粗榧中的山茶苷A  

Quickly purification of camellianin A from Cephaltaxus sinensisby preparative HPLC

作　　者：蒋丹[1,2] 张永谦[1] 戴荣继[1] 李亚秋[2] 赵伟[2] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100081 [2]北京联合大学生物化学工程学院,北京100023

出　　处：《分析仪器》2012年第5期35-38,共4页Analytical Instrumentation

摘　　要：本实验是要研究粗榧中山茶苷A的分离方法,寻找一种高效液相色谱制备分离山茶苷A的快速简便方法,为粗榧药材及其黄酮类提取物的研究和应用奠定基础。实验采用的方法是用大孔吸附树脂柱色谱和硅胶柱色谱粗分,乙醇浓度梯度洗脱,得到山茶苷A粗品,用制备型高效液相色谱进一步分离纯化。结果表明,经制备色谱分离纯化的样品纯度达98%以上,光谱学初步鉴定为山茶苷A(芹菜素-5-O[α-L-吡喃鼠李糖基-(1→4)-β-D-6-O-乙酰基-吡喃葡萄糖苷])。本研究建立了利用高效液相色谱法,从山茶苷A粗品中分离制备高纯度山茶苷A的色谱方法,此方法简便易行。Objective： To establish a purification method of Camellianin A from Cephaltaxus sinensis. Methods： After extracted with ethanol, the extract of Cephaltaxus sinensis was isolated by macroporous adsorptive resins column chromatography and silica gel column chromatography, then purified by preparative HPLC. Results： The purity of product was greater than 98% by analytical HPLC, its structure was determined by chemical and spectroscopic methods as Camellianin A. Conclusion A purification method for preparation of Camellianin A from Cephaltaxus sinensis by preparative HPLC was established. The method is simple with lower cost, and higher sample purity.

关 键 词：制备高效液相色谱 粗榧 山茶苷A 分离纯化 

分 类 号：TQ914[化学工程] O657.72[理学—分析化学]