荷移络合物分光光度法测定头孢哌酮钠的研究  

Study on charge-transfer complex in the spectrophotometric determination of cefoperazone sodium

作　　者：宋健玲[1] 谢鲜梅[1] 

机构地区：[1]太原理工大学化学化工学院,太原030024

出　　处：《分析仪器》2012年第5期44-47,共4页Analytical Instrumentation

基　　金：国家自然科学基金(No.50872086);山西省自然科学基金(No.2008011018)资助项目

摘　　要：研究了头孢哌酮钠与对苯醌的荷移反应。确定了形成电荷转移络合物的最佳反应条件。在硼砂介质中,电子给予体头孢哌酮钠与电子接受体对苯醌于室温下可形成1:1的荷移络合物,络合物的最大吸收波长为610.5nm;表观摩尔吸光系数ε=1.3×10~4L·mol^(-1)·cm^(-1);线性范围为2～64μg/mL;相关系数为r=0.9986,并对形成荷移络合物的机理进行了探讨,应用拟定的方法对样品头孢哌酮钠注射液进行了含量测定,回收率为98.37～100.7%;RSD≤2.09%。The charge-transfer reaction between cefoperazone sodium and p-benzoquinone has been studied spectrophotometrically. The optimization of experimental conditions on the formation of charge transfer complex was performed. At room temperature, cefoperazone sodium reacts with p-benzoquinone in borax buffer solution and forms a 1 ： 1 complex with apparentmolar absorptivity of 1.3× 10^4 L · mol^-1 · cm^-1 at 610.5nm, Beer＇s law is obeyed in the range of 2- 64μg/mL of cefoperazone sodium with a correlation coefficient of 0. 9986. The proposed method was applied to the determination of cefoperazone sodium for injection and satisfactory results have been obtained. The recovery is 98.37-100.7% and the relative standard deviation is≤2.09%.

关 键 词：荷移反应 分光光度法 头孢哌酮钠 对苯醌 

分 类 号：TQ460.7[化学工程—制药化工] O657.3[理学—分析化学]