黄芪多糖(APS)拮抗铅镉联合致鸡脾脏细胞凋亡作用的研究  

Rivalry Effect of Astragalus Polysaccharides(APS) Against Splenocytes Apoptosis by Lead Cadmium Take Care,Toxicosis in Chicken

作　　者：费东亮[1] 苏禹刚[1] 肖银霞[1] 白刃 王令[1] 吴思思[3] 

机构地区：[1]辽宁医学院畜牧兽医学院,辽宁锦州121000 [2]沈阳市沈北新区动物卫生监督管理局,沈阳110121 [3]辽宁医学院医疗学院,辽宁锦州121000

出　　处：《中国农学通报》2012年第26期51-54,共4页Chinese Agricultural Science Bulletin

基　　金：辽宁医学院横向课题"促生长中药饲料添加剂的研制"(K00446)

摘　　要：为了探讨黄芪多糖注射液对铅镉联合致鸡脾脏细胞凋亡的拮抗作用。采用TUNEL法、RT-PCR和DNAladder方法检测分别对对照组、铅镉组和黄芪多糖-铅镉组的鸡脾细胞凋亡率和脾淋巴细胞凋亡基因Bcl-2mRNA转录水平。结果表明:与对照组比较,试验组的细胞凋亡率明显升高,Bcl-2mRNA表达水平降低;试验组和对照组差异显著或差异极显著,并且黄芪多糖-铅镉组数据介于对照组和铅镉组之间。试验结果显示:黄芪多糖注射液对铅镉联合致鸡脏脾细胞凋亡具有明显的拮抗作用。The purpose of this study was to explore APS against splenocytes apoptosis by lead cadmium toxicosis in chicken.Apoptosis rate of splenocytes and transcriptional level of Bcl-2 mRNA were detected by TUNEL method and RT-PCR in control,lead cadmium and APS-lead cadmium group.The results showed that：there were higher apoptotic rate and lower transcriptional level of Bcl-2 mRNA,compared with the controls;There were significant or very significant difference in control and experimental groups,and the data of APS-lead cadmium group was between control and lead cadmium groups.It suggested that APS could resisted splenocytes apoptosis by lead cadmium toxicosis significantly.

关 键 词：黄芪多糖 拮抗 铅镉 脾细胞凋亡 

分 类 号：S856.5[农业科学—临床兽医学]