梅花鹿鹿茸表皮生长因子基因原核表达载体的构建及鉴定  

Construction and Identification of Prokaryotic Expression Plasmid for Epidermal Growth Factor Gene of Sika Deer

作　　者：苏凤艳[1] 宗颖[1] 何忠梅[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《经济动物学报》2012年第3期129-132,共4页Journal of Economic Animal

基　　金：吉林省教育厅项目[2009第(66)号];吉林省自然科学基金项目(201115194)

摘　　要：通过T-A克隆技术,成功地构建了克隆载体pMD-18T-EGF,用EcoRⅠ和HindⅢ双酶切克隆质粒pMD-18T-EGF后,回收EGF基因,并将此基因定向克隆至相同双酶切回收后的pET32a原核表达载体中,获得重组质粒pET32a-EGF。经限制性内切酶分析和PCR鉴定,结果表明:成功地构建了原核重组质粒。The clone vector pMD-18T-EGF was constructed successfully by T-A clone technique and was digested with EcoR Ⅰ and HindⅢ, and the prokaryotic expression vector pE332a was also cut, then the gene was cloned into the vector. Finally a recombinant plasmid named pET32a-EGF was obtained, and identified by PCR, restriction enzyme analysis and sequencing.

关 键 词：梅花鹿 鹿茸 表皮生长因子 原核表达载体 

分 类 号：Q78[生物学—分子生物学]