RP-HPLC法同时测定人体血清及脑脊液中万古霉素的含量  

Determination of Vancomycin in Human Serum and Cerebrospinal Fluid by RP-HPLC

作　　者：王佳庆[1] 梅升辉[1] 

机构地区：[1]首都医科大学附属北京天坛医院药剂科,北京100050

出　　处：《药品评价》2012年第23期22-25,共4页Drug Evaluation

基　　金：国家级863计划重大项目"神经系统疾病的脑脊液净化治疗技术";编号:2007AA02Z453

摘　　要：目的:建立人体血清及脑脊液样品中万古霉素浓度的高效液相色谱测定法。方法:以外标法定量,采用AglientZORBAXSB-C18色谱柱(4.6×250mm,5μm),流动相为30 mM磷酸二氢钾缓冲溶液-乙腈(91:9,pH3.2),流速1.1ml/min,检测波长236nm,柱温20℃。结果:万古霉素在5～200μg/mL血清浓度范围内线性关系良好(r=0.9981),最低定量限为5μg/mL,提取回收率在23.40～47.36%;日内RSD为3.57%、0.97%、0.46%(n=5),日间RSD为4.49%、0.90%、4.10%(n=5);在10～2000μg/mL脑脊液浓度范围内线性关系良好(r=0.9997),最低定量限为10μg/mL,提取回收率在48.61～53.20%;日内RSD为3.10%、6.34%、4.06%(n=5),日间RSD为19.47%、0.87%、2.53%(n=5)。结论:本方法快速,准确,具有创新性。Objective： To establish an HPLC method to determine the concentration of vancomycin in human serum and cerebrospinal fluid.Method： Using the external standard method,the chromatography was preformed on an Aglient ZORBAX SB-C18（4.6×250mm,5μm） column with the mobile phase consisting of 30 mM potassium dihydrogen phosphate buffer solution-acetonitrile（91：9,pH3.2） with the flow rate 1.1 ml/min.UV detection was set to 236 nm and the column temperature was 20 ℃.Results： In the serum,the calibration curve was linear within the range 5～200μg/mL（r=0.9981）.The limit of test was 5μg/mL with the extraction recovery of 23.40～47.36%,and intra-day and inter-day RSD were 3.57%,0.97%,0.46%（n=5） and 4.49%,0.90%,4.10%（n=5）.In the cerebrospinal fluid,the calibration curve was linear within the range 10～2000μg/mL（r=0.9997）.The limit of test was 10μg/mL with the extraction recovery of 48.61～53.20%,and intra-day and inter-day RSD were 3.10%,6.34%,4.06%（n=5） and 19.47%,0.87%,2.53%（n=5）.Conclusion： The method is rapid,accurate and innovative.

关 键 词：高效液相色谱 万古霉素 血药浓度 脑脊液 

分 类 号：R96[医药卫生—药理学]