壮药战骨种质资源遗传多样性ISSR分析  

ISSR analysis on genetic diversity for germplasm resources of Premna fulva in Zhuang medicine

作　　者：熊忠臣[1] 史艳财[1] 漆小雪[1] 邹蓉[1] 蒋运生[1] 韦记青[1] 

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西桂林541006

出　　处：《中草药》2012年第10期2040-2044,共5页Chinese Traditional and Herbal Drugs

基　　金：广西科技攻关项目(桂科重1298001-1-5);广西自然科学基金项目(0991227);广西科技创新能力建设项目(0992028-10);广西植物研究所基本业务费项目(桂植业12011)

摘　　要：目的对壮药战骨种质资源的遗传多样性进行研究。方法采用ISSR分子标记技术分析9个战骨种群的遗传多样性。结果 13条引物共检测到73个位点,其中65个位点具有多态性,多态位点百分率(PPL)为89.04%。战骨总的Nei’s基因多样度指数(H)为0.226 5,Shannon’s多样性信息指数(I)为0.357 2,各种群的平均H、I为0.147 6、0.221 9。种群间基因分化系数(Gst)为0.348 4,基因流(Nm)为0.935 1。9个种群可聚类划分为2个大种群组。结论战骨种群遗传多样性为中等偏低水平;战骨种群的遗传变异主要存在于种群内;战骨的生物学特性和分布区域的片段化是导致种群间出现一定的遗传分化和种群遗传多样性较低的主要原因。Objective To investigate the genetic diversity for germplasm resources of Premna fulva in Zhuang medicine.Methods The genetic diversity of the nine populations of P.fulva was analyzed by ISSR molecular markers.Results Thirteen primers produced 73 sites,among which 65 were polymorphic sites and Nei’s genetic diversity index（H） and Shannon’s information index（I） were 0.226 5 and 0.357 2,respectively,while average H and I at population level were 0.147 6 and 0.221 9.Genetic differentiation coefficient（Gst） and gene flow（Nm） were 0.348 4 and 0.935 1 within the population levels.Cluster analysis indicated that the nine populations could be divided into two groups.Conclusion The genetic diversity among the populations of P.fulva is at lower-middle level.The genetic variance of P.fulva mainly exists among the populations.The high genetic variance among the populations and the low genetic diversity within populations could be attributed to the biological characteristics of P.fulva and its region segment.

关 键 词：战骨 种质资源 遗传多样性 Nei’s基因多样度指数 Shannon’s多样性信息指数 ISSR 

分 类 号：R282.7[医药卫生—中药学]